BtaINT0088436 @ bosTau6
Intron Retention
Gene
ENSBTAG00000021024 | MACF1
Description
microtubule-actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664]
Coordinates
chr3:107336258-107336904:-
Coord C1 exon
chr3:107336798-107336904
Coord A exon
chr3:107336378-107336797
Coord C2 exon
chr3:107336258-107336377
Length
420 bp
Sequences
Splice sites
5' ss Seq
ACGGTAAAA
5' ss Score
6.53
3' ss Seq
TGTCTCCCTTTTGGCAATAGGAA
3' ss Score
8.32
Exon sequences
Seq C1 exon
ATGTGGATGTGCCATCTCCAGATGAGAAGTCTGTAATCACTTATGTGTCTTCAATTTATGATGCCTTCCCTAAAGTCCCCGAGGGTGGAGACGGGATCAGTGCTACG
Seq A exon
GTAAAAGAACATTTTCCCAAAATACCTTCTGGCTGTGATTGCTTTGGGTGGAGTGTGTTGATTAGACTGTGTTTCTACAGCACACAGTGTGTGCATAGCACTTTTGTACAGAGCAGACTGAATCCATATCGTCTTTATACATGACTGTTGTCCTTGGTCCATGGGAATGAATGTAGAGAGCGCTAATTTTGTAGCGTCCAGCATAGGCTATTCTAGGTGTCAGGAAATTGAATCCCATCTTGAGAGTCCTTGAGGAGCTTTGAAACTGGGACTCTGAGTGTTAAACTGAGTGGCTGTGTCTGTGTCAGTAATTAAAGGAAAGGGCTTTACGTGGTTGGGGGTGGCCTGAAATACTTCCTCCTGAGCTTGGCTTGCACACTGCTAATCAGGGCGTGTGTGGTGTCTCCCTTTTGGCAATAG
Seq C2 exon
GAAGTGGACGCCAGGTGGCAAGAATACCAAAGCCGAGTGGACTCCCTGATTCCCTGGATCAAACAGCACACAATACTGATGTCAGATAAATCCTTTCCCCAGAACCCTGTTGAACTAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021024:ENSBTAT00000033270:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.028 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0030726=CH=PD(22.5=63.9)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGATGTGCCATCTCCAGAT
R:
TTAGTTCAACAGGGTTCTGGGG
Band lengths:
222-642
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]