DreINT0091866 @ danRer10
Intron Retention
Gene
ENSDARG00000028533 | macf1a
Description
microtubule-actin crosslinking factor 1a [Source:ZFIN;Acc:ZDB-GENE-030131-3606]
Coordinates
chr19:35604388-35606344:-
Coord C1 exon
chr19:35606238-35606344
Coord A exon
chr19:35604508-35606237
Coord C2 exon
chr19:35604388-35604507
Length
1730 bp
Sequences
Splice sites
5' ss Seq
CATGTACGA
5' ss Score
3.06
3' ss Seq
ATGTGGTTTCTATTGTTCAGGAG
3' ss Score
7.12
Exon sequences
Seq C1 exon
ATGTTGATGTGCCGTCACCAGATGAGAAATCTGTCATCACGTACGTCTCCTCCATCTACGACGCTTTCCCCAAGATCCCAGAGGGTGGAGAAGGCATTGCAGCTCAT
Seq A exon
GTACGATCACTTCAGAGTGCACTCACTGTCTGCTCAATTATGAAAAAAATCATTATCACGATTATATATAATATGTATATATGTATGTATGTATGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATATATTTCATAGATCCTGAAATTTCTAAATTTTCAATACTGAAGCCAAATGGGTTTTATGGGACACCATAAAAACACAATAGAATAAAATATGCAATCAAAGTAAACAGTTTTAAGCATCTTACCAGTACGATTTAAGCTTTATTTTTATTTTAATTATTATTATTAAACATACAAAAGTCTTTCAGGTAAGATTAGTGAGTGATTTTTGTCTTTTATTTGATTAATAATGACAAACACAGTCAGGAATATAGAGCACTGTTTGGATTTATGGTTACACTTGGCTTTTTATTCTCAACTCTTTATTAATTTATTACATAACAAAGAGGATTAGTTTAAATAATGACTAAGCAATGCTTTTTACACATTTTTGCAAGTATTTGACCGTTTAGCATGCACCTTAGATGTTTGATGGCATAATCGTGTATATAAAAAATAATCAAACAGGGAGAGTCTGAAAACCTTTTTGTACAAGGAAACAGTTGTTTCAACTGTTCTTTCACATCTGCAGCTGACCGTCAGAACAGCAGAAACTGTTGCTACTTCACAAACGTCACTTTAGCGCTAGTATTTGAATGATTCTCTAGCATAATGTCTAAAATGATGACAAACAGGTGATTCTGCTCAGATTATAAGGCTGAACCGCATGAAATGCCATCAGTTTTCAGAGATTTCCCAGTTTTTTTTCTGTTGCAATTTGGAGATCATAATAATTAATCCCAAAAAAGCCAGAGCAGAACAAACCCTAGCAGATTACTATGGTATAAATATAGCATTACAAAATACAAAAGAGAGAGATCGACTTAGAATGCCACTTACCTGTGGCCATCTGTACCATCTGCAATGATTTGTTCAGCTGTAGTTTGAAACGTACTCTGAAAAAATGCTGTTTTTTCTCTCTTAAGGACTTATTATGTATTCTGTATTATAATTATTATTATTATTATTATTATGTATTATGTATTATTAACGTCTCTTAAGGACGTTATTATTATACCGTCTTTGCTCTGCTCAGTATGGGCGAATCTCCAAAATGATGAATATTTAAAATAGGCAATATGCTTATAAATAAACTGCATAGTTGCAATCTAAACAACTACAATCTCGTCTGAAAAAAGTCTCAAAAGTACATTATGTTGTCCAACAGCTGCAATGTTTGTCAAACTGTAATTCTCGTGTGGGAGGAGTAATACACAGTGGTGAAGAGGCTGTATGAATGCTGATATTCCTTCATATTTCTCACTGCTAACAGCTAATCAAATTCAAGAACCAGACAGAACTGTTGTATAAATTCATGTTCTATATTTCAAAAGTCATGTTACATGATTTTACTGATTTCCACTTGGAATTAAGCTGATACAAACAAATGGACTGCTGGAAAGGAGGCAGGATATGACGCAATAAATTTTGATCTCAATTCATGTTTTTTGATAATCATTAGAGGCACTTAAATTTGAGTAAGTGCGCAGCCCTACTATCTGCACCTTCTATGCTCATGCCATTTAAACTCTTGGTCTAATATGTGGTTTCTATTGTTCAG
Seq C2 exon
GAGGTTGACCAGCGATGGACGGAATACCAGTCTGGCTTTTCTTCTCTGCTGCAGTGGACCAGGCAACACACAACCCTCATGGCCAATAGGAGCTTCCCCCACAATCCTGTGGAACTCAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000028533:ENSDART00000146394:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.056 A=NA C2=0.050
Domain overlap (PFAM):
C1:
PF0030726=CH=PD(22.5=63.9)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGTTGATGTGCCGTCACCAG
R:
GGATTGTGGGGGAAGCTCCTA
Band lengths:
214-1944
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]