BtaINT0097133 @ bosTau6
Intron Retention
Gene
ENSBTAG00000015988 | MYH11
Description
myosin-11 [Source:RefSeq peptide;Acc:NP_001095597]
Coordinates
chr25:14245913-14247559:-
Coord C1 exon
chr25:14247353-14247559
Coord A exon
chr25:14246051-14247352
Coord C2 exon
chr25:14245913-14246050
Length
1302 bp
Sequences
Splice sites
5' ss Seq
CTGGTAAGG
5' ss Score
9.68
3' ss Seq
CTTTTCCCTCCCCCACAAAGGAC
3' ss Score
7.83
Exon sequences
Seq C1 exon
CTGACAGAGGAGAAGAACCTGCTGCAGGAGCAGCTGCAGGCAGAGACGGAGCTGTACGCCGAGGCCGAGGAGATGCGGGTCCGGCTGGCAGCCAAGAAGCAGGAGCTGGAGGAGATCCTGCACGAGATGGAGGCCCGCCTGGAGGAGGAGGAAGACCGGAGCCAGCAGCTGCAGGCAGAACGGAAGAAGATGGCCCAGCAGATGCTG
Seq A exon
GTAAGGCGCTGCGGGGGCTGCCCTCGGTGGTGCCGAAACCCTTGACTCTCGCTGCAGACCTGTGAGGTGCATCTGATGATGTGTGTCCCCACTGGACTGAGATAGAGACTCAGGCTTGGAGAGGAGCAGCCCTCTGCCTACACAGGTCTCCTCAGGAGCCTGCTATGTCCTGCTGCCCTGCCTTCCATGCGCAAACACGTTTCTCCTAATAGCCACCCTGTGTGAGGGGTAATTGTATCAGTAAGCTACTGCTGCAAAACAAAAAGCACCAAGACTCAGCAGCTTAGAAATAAGAAGCATTTAGGACTTCTCTGCTGGACCAGTAGTTAAGAATCTGCCTGCCAGTGCAGGGGGACACAGGTTCGCTCCCTGGCCCAGGAAGATCCCACGTGTTGTGGGGCCTGTGTGCCACAACTACTGAGCCGCACTCCAGAGCCCACGAGCTGTAATTGCCAAGCCCACATGCCAAAGCTGCTGAAGTCCACATGCCTAGAGCCTGTGCTCTGCAGCAAGAGAACCCACTGTGATGAGAAGCCCGCACACCACAACCAGAGAAAGCTGGAACCAGCACACCCGAAAATAAATAAATGTAAAAATATATATTTTTTGGTAAATGAAATACTAAACATTTATCTCCCACAGTCATGACTCTGAGAGTGGCTTAGCTGGATGATTCTGGCTTAGAGCTTCTCATGAGATTGCAGTTGTGATGTCAGCCTGGGCTGCAGTCTTCTGAAGGCTTGACTGGGCCTTGAGGATCCCCTTCCAGGGGTGAGTGAGCCGTCACTCACATGGCTGTAGGCAGGAGACCTCAGTTCCTTGTTCCGTGGGCTTCTCCACAGGGCTGCTCGGGTGTCCTCACAGTATGGCAGCTTGCTTTCCCTGGACAGAGCGAACGGAGGGAAAACAGGGGAGGAAGCTGCAATGCCTCTTATAGCCTAGTCTCTGACTTACACACGCCATCGCTTCCTCTTGATTTTGTTAGAAGCAAGTCATTAAGCCCAGCTGACACTCAAGGGGAGAAGAATTACACTCCACCTGTTGAAGGGAAGAGGATCAAAGAATTTGTGGACATATTTTTAAAACCACCATGGTATCTATTCTCCCCACTTTACAGATGAGGAGACTGAGGCTCCACAAAGCAAAGTGGCTTACCCAAACCTTTTCTTTCTGATGAAAAGAGACTGCAGAGCACTGCAGGGAGCCATGAGGCCCAGCCTCTGTATCCATGAGTCCCCAGAGCTCCGGGAGAGTAGATTTTGGGTTAAACAAGGCTGTAATTCTTTTCCCTCCCCCACAAAG
Seq C2 exon
GACCTGGAGGAACAGCTGGAGGAAGAGGAAGCTGCCAGGCAGAAGCTACAACTTGAGAAGGTCACAGCTGAGGCCAAGATCAAGAAACTGGAGGATGATATTCTGGTCATGGATGATCAGAACAATAAACTGTCAAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000015988:ENSBTAT00000035455:24
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.507 A=NA C2=0.500
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=PU(8.8=18.8)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(30.4=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGACAGAGGAGAAGAACCTGC
R:
TGACAGTTTATTGTTCTGATCATCCA
Band lengths:
342-1644
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]