BtaINT0120953 @ bosTau6
Intron Retention
Gene
ENSBTAG00000018928 | QARS
Description
glutamine--tRNA ligase [Source:RefSeq peptide;Acc:NP_001029640]
Coordinates
chr22:51489964-51490356:+
Coord C1 exon
chr22:51489964-51490123
Coord A exon
chr22:51490124-51490270
Coord C2 exon
chr22:51490271-51490356
Length
147 bp
Sequences
Splice sites
5' ss Seq
AAGGTGTGT
5' ss Score
6.64
3' ss Seq
TGAGTTGGGGGTGGGGTGAAGGG
3' ss Score
-20.26
Exon sequences
Seq C1 exon
GTGGGAGTGACAGTGGCACAGACCACAATGGAACCACATCTGCTAGAAGCCTGTGTGCGTGATGTGCTGAATGACACAGCCCCTCGGGCCATGGCTGTGCTGGAGCCGTTACAGGTGGTCATCACCAATTTTCCTGCCACCAAGGTAAGTCTCCTTCAAG
Seq A exon
GTGTGTGTGGGGGGGGGAGCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGGTGAGTTGGGGGTGGGGTGAA
Seq C2 exon
GGGTCCGGTTTGCTCCTGAGCTCAGTCTGAGGACCCATCATCCCTTCTTCAAACCAGTCTCTGCCAGACATAGGGGTGGAAGAAGC
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000018928:ENSBTAT00000025191:18
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.276
Domain overlap (PFAM):
C1:
PF0074916=tRNA-synt_1c=PD(8.0=44.4),PF0395013=tRNA-synt_1c_C=PU(14.4=50.0)
A:
NA
C2:
PF0395013=tRNA-synt_1c_C=FE(15.0=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGGAGTGACAGTGGCACAG
R:
TCTTCCACCCCTATGTCTGGC
Band lengths:
242-389
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]