BtaINT0122624 @ bosTau6
Intron Retention
Gene
ENSBTAG00000015537 | RASAL2
Description
RAS protein activator like 2 [Source:HGNC Symbol;Acc:HGNC:9874]
Coordinates
chr16:61277023-61279040:+
Coord C1 exon
chr16:61277023-61277176
Coord A exon
chr16:61277177-61278941
Coord C2 exon
chr16:61278942-61279040
Length
1765 bp
Sequences
Splice sites
5' ss Seq
GAGGTAGGA
5' ss Score
8.24
3' ss Seq
CTCTAATCTTTATGTTGAAGGTT
3' ss Score
6.33
Exon sequences
Seq C1 exon
GTCTCGTGGGCTGCCTAAACTAAAAGAGTCCCGTTCTCATGAATCTTTGCTGAGCCCATGCAGTGCCGTGGAATGTCTGGATCTCGGTAGAGGGGAGCCTGTGTCGGTAAAGCCACTCCATAGCAGCATCCTTGGACAAGACTTCTGCTTTGAG
Seq A exon
GTAGGAAAGTCCAGGAAAAACAAAGAGTAGTAAGACAGCAAAGAGATTTTCTATCCAATATTCTTATGATTGCATTACTGCATTATTGATGATTTGATCATTTTAACTGGTTTTCAGTTACTGAGAATTACCTACTTACAGGGGAAAAATTAGAAATCTGCTAATCTAATTATGTTGGTAATTAAAGTTATTAATGATGGTTTCTCATTCCTTTTGGCTTTGTCTGCTTTCAAAATAAATGAATTCTTAGTACATTCTCAGCAGAGATCACAAGATTAATACTAAATTATTGTTAGTATTTTAATCTTTTGTTATTGAGAAAGATATCACACAGTACGTTACTATTTTTCTTCTGAAAACCTAAGTCCCCAATTTTAATAATTCTTTGAGGGAAAAATACCAAAACCATACTAACTAAAACCTGAGTGGTTCAAGATCTGGGATCATGTCTTCTTTATTTTTATAATTTCTCTGCACTTAGCTAGCTTCCTGGCACATTGCATGTGCCAATTTCTTCAGTGAACAAACTGCCCTTTACCAGGAAATTATCACTTTTTATAAGGTAATTTTCCCCTCCCAAACAATGTATACAATTGGCCATTTGCAAATTTAATTTCAAAACCATCTGTGTACTTGTTAGCATTATGATTTAATTAATTAGCACAGACTTACAAAATTACTAATTATGAGTCCTATGTTAGATGTTGTAAAAGTTGAAAGATATATCTGATATATATTTTTTATATATATCTTTCTTAGGGACCTGCCCTATCCAACAGAAATACAGTGAAAGCCATGTGTGTGATTTTAAATTTTATTAAACCCAAATGTTACCACTTCCAACATGTAGCTAAAATAAAATTATCAACTTTCCACCTTCTTTTTTTAAAACTAAGTCTTCAAAATCCGATGTGTGTTACACTTACCGCACATCTCATTTGGACTAGTTGTTTTTCAGGTGCTCACTACCACCTGTTGTTAGTAGCTGCCATGTTAGACAGAACGTAACGTCTGATCTAATTCACTAGGTTATAAACTGAGACTAAGTTACTATGTGAGCCACGGTAGCAATTTGGACAGGTCATTTATTGGTGTTCTTTTTTATAAAAGGTTTGCCATATTTGTGGAAATTAGTTCAACAGATTGTATGCCGAGACAGGTGGTAGGTACTAGACATAGAGACAACATACTTCATTTTTAAAGGACATTGATAGTAATATTCAAACTAACCTAATGACTGGAAATCAACTGACTGGTTGCCTAATAGAAGAGAAGTGGGTTCCAGAGTAATTAATTCAACACTACGAACTCTTTTCAATTTCATGTAGTAGCCTTCTGTAATATTATAATGTGAAAGCATTCCCAAGAAATAAGCTGTTAGAGATCTTCTTCCCTTTTTAATTATTTTTTTATTAACTCACTCAGAATTGAGGATATTTATTTGCAGGTAATTTTTTAAAATATTAATTTAATTAGAGGTTTTCAGTTTTGTTCTTTCTAAACTATAGGCCAGTTTTCGGTTCCTAAGCTGAATTCTCAGTGACCTTTGTCCTTACCTGGAGAAAAGTATAGATGCTAAGTAGATAAAAAGCAAAAAGTGAATGTAGACGCACACATAGATATCACAAAGCCAGCAAATATCATTTTGGTTACCGTATCATTTTGGGTTCACAGCATATCCCTAACTCTTAAATGATACCCCTAGGAAGATAGAGCTCCCAAGCCCTATGTTTCCTTTTCCTTATCTCTAATCTTTATGTTGAAG
Seq C2 exon
GTTACCTACTTAAGTGGAAGTAAATGCTTCAGTTGTAACTCTGCTTCAGAGAGAGATAAGTGGATGGAAAACCTGCGCAGGACAGTTCAACCAAATAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000015537:ENSBTAT00000020658:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.192 A=NA C2=0.121
Domain overlap (PFAM):
C1:
PF0016924=PH=FE(43.2=100)
A:
NA
C2:
PF0016924=PH=PD(24.6=87.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTCGTGGGCTGCCTAAACTA
R:
TTTGGTTGAACTGTCCTGCGC
Band lengths:
248-2013
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development