MmuINT0132045 @ mm10
Intron Retention
Gene
ENSMUSG00000070565 | Rasal2
Description
RAS protein activator like 2 [Source:MGI Symbol;Acc:MGI:2443881]
Coordinates
chr1:157177646-157179252:-
Coord C1 exon
chr1:157179099-157179252
Coord A exon
chr1:157177745-157179098
Coord C2 exon
chr1:157177646-157177744
Length
1354 bp
Sequences
Splice sites
5' ss Seq
GAGGTAAAA
5' ss Score
7.3
3' ss Seq
CTCTAATCTTTGTGTTGAAGGTC
3' ss Score
6.47
Exon sequences
Seq C1 exon
GTCTCGAGGTCTGCCTAAACTGAAAGAGTCGCGTTCCCATGAGTCCCTGCTGAGCCCATGCAGTGCAGTGGAGTGTCTGGATCTAGGTAGAGGAGAGCCAGTCTCAGTGAAGCCACTGCACAGTAGCATCCTCGGACAGGACTTCTGCTTCGAG
Seq A exon
GTAAAACATAGGAAGAAGGGTAATACTTTAGCAGTTTCAATGTTTCTGATTTGATTATCTTAACTGGTTTTCAATTACTAAAGTTTTTTTTCTTAAAAGAGAACAGTTAAAAATCTGTTAATAGAATTATATTTGTAATTGAAGTTATTCATAATTCTCATGCCTTTGCTTTCAAAAACTTCAGCACTTAGTACATTCTCAGAATAGATTACAAGATGAACAATAGCTTCTCCAACTAGTATGCTAATCTTTAGTATTGATGTCATATACTGGTATTTAATCCTTAATTTTAAGGGAATTTTGAGAAAATCCACTAAAATCACAATTTTATAACTATAACATGAGCAATTCTTGACCGAGGTCCATACTTCGTTTGTTTTTTTAATCTCCTGCATGTTCTTAGATCAATAGCCTCATTTCCGGTCTTCACTAATCATCTGAATCTAGGAACTGCCATTTCAGACGCTGCGCACTGTCTGATCTGATTCATTGTATTAATGAACTGAGAATGACAGACTACAGCAGTTTTAGCAGATCATTTATATGTGTGTTTATAATTAATAAGAATAAAGTTGTTGTTATTATAATCATTATTAAAAATCTCTTGAACTTTTATCTGTGGAGCTCAGTTCAGTAGGCTTCACTGTGGCCATAGTGTATATGTCCAGTGTTATACATGACTGGAAGTCAGTGGGTTGGTTGCCTCCAGGCGGAGAAGTGGCTTCCAGAGAGTGATTCAGTTGTGCTGATGCCTTTCAACTTCCTTTATAGCCTTCTGTAGCATTATAATGAAATAATCAGAAGACATCAAACGATTTTACAAACATTTTCACTTTCTAGTTAATTTTTAAAAAATATGTATTTATTTATTTATTATAAGTACACTGTAGCTGTCTTCACATACCTCAGAAGAATGCATCAGATCTCATTATAGATGGTTGTGAGCCACCGTGTGGTTGCTAGGATTTGAACTTAGGACCTCCAGAAGAGCAGTCAGTGCTCTTAAACGCTGAGCCATCTCTCCAGCCCTTTTAGTTTATTTTATTAGCTCAAATGAACATATTCATTTACAAGTGATTTTAGATGTGTCCTTAAATGTTTTTAGTATTTTTCTTAACCCTAGGCAAGCTAAACTGTGAATGATCATTGATCTCAGAGCTCAGACGTGTGCATGCTACTTAGAGATTACATTTTAAGTAAAGATATGACAAAGCTAAAACAACTGTTTTAGTTTTACATGGTAAGATTTGAGTTCCAGACATGTCCTTAATTCTTTAAAAACAAAATAGAAATTAGAAGATAAATCACACCCATACCCAGTCTTACATCATCATCTCTAATCTTTGTGTTGAAG
Seq C2 exon
GTCACCTACTTAAGTGGAAGTAAATGCTTTAGCTGTAGCTCTGCCTCCGAGAGAGATAAGTGGATGGAAAACCTTCGAAGGACCGTTCAGCCAAATAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000070565:ENSMUST00000078308:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.202 A=NA C2=0.117
Domain overlap (PFAM):
C1:
PF0016924=PH=FE(22.1=100)
A:
NA
C2:
PF0016924=PH=PD(12.6=87.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGTCTGCCTAAACTGAAAGAGT
R:
TTATTTGGCTGAACGGTCCTTCG
Band lengths:
246-1600
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types