DreINT0124804 @ danRer10
Intron Retention
Gene
ENSDARG00000036257 | rasal2
Description
RAS protein activator like 2 [Source:ZFIN;Acc:ZDB-GENE-070912-180]
Coordinates
chr2:35852896-35854520:+
Coord C1 exon
chr2:35852896-35853049
Coord A exon
chr2:35853050-35854421
Coord C2 exon
chr2:35854422-35854520
Length
1372 bp
Sequences
Splice sites
5' ss Seq
GAGGTACAG
5' ss Score
6.62
3' ss Seq
TGTTCTTACTTCTCATGTAGGTG
3' ss Score
9.77
Exon sequences
Seq C1 exon
GTCACGAGGCCTGCCCAAATTGAAGGAGTCCTGTTCCCATGAGTCTTTGCTGAGTCCTGGCAGCGCTGTTGAAGCTCTGGATCTGAGTATGGAGGAGGATGTCTACATCAAACCTTTACACAGCAGCATCCTAGGACAAGATTTCTGCTTTGAG
Seq A exon
GTACAGACTTTGCCTTTTCGAGTTGTTCCCTTAATCATATTAATATTTATCTTTACGCTAGCATTAAGAGGTTGGGGAACTGTAAAAAAAAAAAAATAATTAAAGACAAATAAAAATATTGAAGTATAAATTATAATTTTTTGTATTATTAGTATTTTTAAACGTTATGTTTTTTTCTGTGACGTTTAGGTTAATTATATTTAGATATAAGCAGATATTTCTTGAGTGTTCAGTGTCATAAATATATAATGTTTATATTTACAATATATACGATCCTCTGATTTTTAGGACTAGTTGATCATATACAATGTTTTAAACCACCTTTTGTTTTGAAATAGAATAGAATAGATTTTAAAAATGTCTTGTCAGGATTAATATGACACATTCTTGCTAAATAAAAAAGTATTTACAATATATTGTTTAATGAAATGTAATCCTCAAATTGTCCTCATACTTTGAAACAGTATTGGATATGAATCATAATGCCTTGAGTAGTTTGTGAACAAATTATTAATTTGTGTCTGACCTTTTGAGGTAATATATATGATTTCACAAAATTATTTCGCTTATTCATTAGCTATTTAGACTCTTGTGCGTTTTAAAACCCCATGTCCAGTAAACATTTTTTGTTAAAGTGACATATAATGTTTCAAAATATGTAACACTAGCTATGTTTTCATCTACCTATTTTTATGTGCATTTTGGATCTGCGCATAAATAAACGGTTGGAGACACCAAGATGCGCAAGCATTTTGTAAATGCGCATATAAAATGTATGCGCATAACTGAGTAGGATAAACTTTTTATTCAATAAGAAAAGATGCACATAAACTGTGATAAACACTTTTACCGGATAAATTCCAGTATGCGCATTGAAAATAGTCATGATTTTGTTATAAGAGATCATGTAATGATAAAAAATGGGTGTGAATGGACAAACCGGTAGACTGAGCCCACTGTAAAACATCTGAAATGTTGGTTTGGTCATTCTAAAATGCCTTAACTGTTTCAGTATTAGTGTTATTGTATTATTATTTACCTCCAGAATCAAGAGCGTCTGTGCTCTGCGTCTGATGCCTTCAACGCCACCGCGCACTCACTGCGTGTCCAAATTGCTTTCTGAGGCGCAGGTCATTTATTAAATGAAGAAAAGATTCACAAAGCTTCTCCTACTGCAGCAAATGCCATTTTTACTGTTGATATTTGGTGCCAGTTAATCAGAAAGTGATGAATTTGTTCCCCTTGACTCATAAAAAAAGCTAATAAACATGAGAACAAACTTGAAAACATCTTAAAGTTTTATCCATTACATAAAATAAAATAATTTACATTAAATAAATGTCCATAATGAATGTTCTTACTTCTCATGTAG
Seq C2 exon
GTGGCGTACTCAGGTGGAAGTAAGTGCTTCAGCTGTTCCTCTGCTGCTGAACGGGACAAATGGATGGAGAACCTCAAAAGAACTGTGCAACCTAATAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000036257:ENSDART00000113489:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.135 A=NA C2=0.114
Domain overlap (PFAM):
C1:
PF0016924=PH=FE(20.3=100)
A:
NA
C2:
PF0016924=PH=PD(11.6=87.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCACGAGGCCTGCCCAAATT
R:
AGGTTGCACAGTTCTTTTGAGGT
Band lengths:
246-1618
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]