DmeEX6022513 @ dm6
Exon Skipping
Gene
FBgn0265042 | Irk1
Description
The gene Inwardly rectifying potassium channel 1 is referred to in FlyBase by the symbol DmelIrk1 (CG44159, FBgn0265042). It is a protein_coding_gene from Dmel. It has 8 annotated transcripts and 8 polypeptides (5 unique). Gene sequence location is 3R:23168317..23185642. Its molecular function is described by: inward rectifier potassium channel activity. It is involved in the biological process described with 6 unique terms, many of which group under: transport; regulation of membrane potential; inorganic ion transmembrane transport; developmental process; locomotory behavior. 19 alleles are reported. The phenotypes of these alleles manifest in: trichogen cell; wing; mesothoracic tergum; wing vein. The phenotypic classes of alleles include: lethal - all die during P-stage; visible; locomotor rhythm defective; viable; some die during pupal stage; partially lethal - majority die.
Coordinates
chr3R:23182946-23185642:+
Coord C1 exon
chr3R:23182946-23183060
Coord A exon
chr3R:23183444-23184164
Coord C2 exon
chr3R:23184223-23185642
Length
721 bp
Sequences
Splice sites
3' ss Seq
ATTTTTTTTTCCTTTTCCAGGAC
3' ss Score
12.9
5' ss Seq
TGGGTGAGT
5' ss Score
8.73
Exon sequences
Seq C1 exon
GTACCGGCAGACACGTTTCAGTTCGCGGCGTGTTCGAAAACGTGTGGTCTTCAAGCACGGCGAGTGCAACGTTGTGCAGGGAAATGTGGCCAAGAGACGGCGTCGCTATCTGCAG
Seq A exon
GACATCTTCACCACCCTGGTTGACGCCCAGTGGCGCTGGACGCTGCTCGTCTTCGCCGCCAGCTTCGTGTTCTCGTGGGCCTTCTTTGGATTCATCTGGTGGATCATCGCCTACGCACACAATGATCTGGAGTACACCAATCTCAAGAACCAGTCACCCGATCTGGTGGCCAACATAACGCACACGGTCTGCGTAACACAGGTCTCCAATATGATGTCCGCCTTCCTGTACTCCGTGGAAACCCAGACGACGATAGGCTATGGTAATCGCTATGTGACGGAGGAGTGCCCGGAGGCGATATTCACGATGTGCATCCAGTGCATCACGGGTGTCTTCATCCAGGCGTTCATGGTGGGCATTGTGTTTGCCAAGTTGTCGCGTCCCAAGAAACGTGCCCAGACGCTGCTCTTCTCGCGCAATGCTGTTATCTGCCACCGGGATGGAGTTCCATGCCTGATGTTCCGTGTGGGAGACATGCGCAAGTCGCACATCATCGAGGCCCATGTGCGGGCCCAGATCATCCGCAAGAAGGTGACAAAGGAGGGCGAGGTGCTGCCCTTCTACCAGCAGGAGTTGCACATCGGAGCCGATGGTGGAGAGGATCGGCTAATGTTCATCTGGCCCACTACCATAGTGCACAAAATTGATAGGAACAGTCCACTGTACATGCTCTCCGCCTCCGATATGCTAAAGGAACGCTTCGAAGTGGTGGTTATGCTGG
Seq C2 exon
AGGGCGTCATCGAGTCCACTGGCATGACCACACAGGCCAGAAGCAGCTACTTGCCCTCGGAGGTGCTGTGGGGCCATCGTTTCGTGAATGTGGTGTCCTTCCGCAAGGAGACCGGCGAATACGAGGTGGACTACACGCTATTCAACAACACCTACGACGTGGACACTCCGCTGTGCAGCGCCAAGCAGCTGGACGAGCTTAAGTCGGAGTACTCGAAGAGCGCCAAGTGTGTGAATGCACCCTTTGCGGATCGCACGCTCTCGGCCAGCTTGTTCCAGCGTATTGCCTCCGCCGCCTCGGTGGATCATCTGGATCCGGCTAGCGACGAATCGCTGGACTCTGGCCGCCTGCAGATACGCTCCCATTCCATACCCAACGGCGTGCTGGCCAGCGAGCTGGAGCCGCTGAATAACAACAAGCATGGCGCTTCGTTCACCATGGGTGGCCTGACCATCACGACGACGGCGCCCAGCATCCCCAACCTATCCAGTATTAACGAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0265042-'11-12,'11-11,12-12=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.469
Domain overlap (PFAM):
C1:
PF0100715=IRK=PU(6.4=56.4)
A:
PF0100715=IRK=FE(69.4=100)
C2:
PF0100715=IRK=PD(23.7=33.3),PF130151=PRKCSH_1=WD(100=35.4)
Main Inclusion Isoform:
FBpp0307580
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
FBpp0307576, FBpp0307577, FBpp0307578, FBpp0307579, FBpp0307581, FBpp0307582
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTACCGGCAGACACGTTTCAG
R:
ATTCACACACTTGGCGCTCTT
Band lengths:
351-1072
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)