MmuEX0025072 @ mm10
Exon Skipping
Gene
ENSMUSG00000032034 | Kcnj5
Description
potassium inwardly-rectifying channel, subfamily J, member 5 [Source:MGI Symbol;Acc:MGI:104755]
Coordinates
chr9:32314707-32342524:-
Coord C1 exon
chr9:32342222-32342524
Coord A exon
chr9:32322081-32323019
Coord C2 exon
chr9:32314707-32317902
Length
939 bp
Sequences
Splice sites
3' ss Seq
TTTAATTCACTGTATCTCAGCTA
3' ss Score
4.65
5' ss Seq
CAGGTAAGA
5' ss Score
10.77
Exon sequences
Seq C1 exon
GGGAAGGCTCAGCTTCCAGTGGGAGCCCTAGGGAACAGGGTGTACTTGGGAGCATGGCAGCATCAGATCACCACTGAAAGAGGTGCCATCCCAGAGATAACTTCATCCTCCAACAGCACCCAGCCATCTTCCCCACGCTGACCACCTAGCTGCCCAACAGCAAAAGCACCCACCACTCTGCCACCGCCTCTCCAAGATGGCTACCTACTGCTGGCCGGGCACTCTGAAACAGCACTTCTTGCTTAAGAGCACATCTGCTTTAAGCATAAAATGAGTGTGTTCTATGCTCCATCTAGCAAGCAG
Seq A exon
CTATGGCCGGTGATTCTAGGAATGCTATGAATCAAGACATGGAGATAGGAGTCACTTCCCAGGACCACAAGAAGATTCCCAAACAGGCTCGGGATTACATCCCCATTGCCACAGACCGCACCCGCCTGCTGACAGAAGGCAAGAAGCCACGCCAGCGCTACATGGAGAAGACTGGCAAGTGCAATGTACACCACGGCAATGTTCAGGAAACCTACCGCTACCTAAGTGACCTCTTCACCACCCTGGTGGACCTCAAATGGCGCTTCAACCTTCTGGTCTTCACCATGGTCTACACCATCACCTGGCTGTTCTTTGGCTTCATTTGGTGGCTCATTGCTTATGTCCGAGGTGATCTGGATCACGTGGGTGACCAAGAGTGGATCCCTTGTGTTGAAAACCTTAGCGGCTTTGTATCTGCTTTCCTGTTCTCCATCGAGACAGAAACAACCATTGGGTATGGCTTCAGAGTCATTACAGAGAAGTGTCCAGAAGGGATCATACTCCTTCTGGTGCAGGCCATTCTGGGCTCGATTGTTAATGCCTTCATGGTGGGGTGCATGTTTGTAAAGATCAGCCAGCCAAAGAAGAGAGCAGAGACCCTAATGTTTTCCAACAATGCCGTCATCTCCATGCGGGATGAGAAGCTGTGCCTCATGTTCCGGGTAGGGGACCTCCGAAACTCCCACATCGTGGAGGCCTCCATCCGTGCCAAGCTTATCAAGTCCCGGCAGACCAAAGAAGGAGAATTCATCCCCTTGAACCAGACCGACATTAATGTGGGCTTTGACACTGGTGACGACCGCCTCTTCCTGGTCTCCCCACTCATCATCTCCCACGAGATCAACGAGAAGAGCCCTTTTTGGGAGATGTCTCGTGCTCAACTGGAACAGGAAGAGTTCGAAGTTGTGGTCATACTAGAAGGAATGGTAGAAGCAACAG
Seq C2 exon
GCATGACTTGCCAAGCACGGAGCTCTTACATGGATACAGAGGTGCTCTGGGGTCACCGATTCACACCAGTTCTCACCTTGGAAAAGGGCTTCTATGAGGTGGACTACAACACTTTCCATGACACCTATGAGACCAACACACCCAGTTGCTGTGCCAAGGAGCTGGCGGAGATGAAGCGGAGCGGTCGGCTCCTCCAGTACCTTCCCAGTCCTCCCTTGCTTGGGGGCTGTGCTGAGGCTGGGAACGAAGCTGAGGCCGAGAAGGATGAAGAGGGTGAGCCCAATGGACTGAGTGTGTCCCAGGCCACCAGGGGCTCAATGTGAAGTCTGTGTGCTACCCAGAGCCTCTTCTTGCCAGACACCGAGGAGCCTGGGAGGCCAGAGAGGCCAATTAGTTGAGCAAGCTTTGTGGGGCCCCAGGAGTATGGAAGCTCTACAGAGAGGAATAGTGTGTCAAGGCCTCACATCTCCTCCTTGGGGCCTCAGAGAAAGTAGCCTTGC
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000032034-'3-5,'3-3,5-5
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion (Ref, Alt. ATG (<=10 exons))
No structure available
Features
Disorder rate (Iupred):
C1=NA A=0.016 C2=0.271
Domain overlap (PFAM):
C1:
NA
A:
PF0100715=IRK=PU(77.3=82.7)
C2:
PF0100715=IRK=PD(22.4=69.4)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGGCACTCTGAAACAGCACTT
R:
TCTTCATCCTTCTCGGCCTCA
Band lengths:
358-1297
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types