DreEX0013092 @ danRer10
Exon Skipping
Gene
ENSDARG00000043313 | ank2b
Description
ankyrin 2b, neuronal [Source:ZFIN;Acc:ZDB-GENE-041010-165]
Coordinates
chr7:57660745-57662162:-
Coord C1 exon
chr7:57662064-57662162
Coord A exon
chr7:57661502-57661603
Coord C2 exon
chr7:57660745-57660942
Length
102 bp
Sequences
Splice sites
3' ss Seq
TGCACTGACCCTCATCCCAGCAG
3' ss Score
4.45
5' ss Seq
AAGGTGAGA
5' ss Score
8.68
Exon sequences
Seq C1 exon
AAAGGGTTCACACCTTTACATGTGGCCTCAAAGTATGGAAGCCTGGAAGTGGCAAAGCTTCTTTTGCAGCGACGGGCACCTCCTGACTCTGCTGGGAAG
Seq A exon
CAGAACGGTCTCACTCCTCTACATGTCGCTGCACACTATGATAACCAGAAGGTGGCACTCCTGCTCTTGGACAAAGGGGCATCTCCTCATGCCACCGCTAAG
Seq C2 exon
AATGGATACACTCCCCTTCATATAGCAGCAAAAAAGAATCAAATGGAAATTGCCACCACACTCCTACAATATGGAGCTGAGACCAACATCCAGACCAAACAGGGGGTGATGCCGATCCATCTGGCGTCCCAGGAGGGACATAGTGAAATGGCGGCTTTGCTTCTGCAGAGAGGAGCCCAAGTCAATGTAACCACTAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000043313_CASSETTE5
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.221 A=0.215 C2=0.161
Domain overlap (PFAM):
C1:
PF127962=Ank_2=PU(28.7=81.8)
A:
PF136371=Ank_4=PU(57.4=91.2)
C2:
PF127962=Ank_2=PD(33.0=47.0),PF0002325=Ank=WD(100=50.0)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGCCTCAAAGTATGGAAGCC
R:
CAGAAGCAAAGCCGCCATTTC
Band lengths:
242-344
Functional annotations
There are 1 annotated functions for this event
PMID: 17178715
Exon 17 (HsaEX0004129) encodes ankyrin repeat 18 of ankyrin-B. Direct Interaction between Ankyrin-B and Na/Ca Exchanger 1 Requires beta-Hairpin Loop Tips of ankyrin Repeats 16-18, and therefore it is expected to be affected by skipping. The function of the isoforms of HsaEX0004129 have not been examined individually.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]