DreEX0013115 @ danRer10
Exon Skipping
Gene
ENSDARG00000061736 | ank3a
Description
ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1]
Coordinates
chr17:20714204-20725385:-
Coord C1 exon
chr17:20725276-20725385
Coord A exon
chr17:20722906-20722938
Coord C2 exon
chr17:20714204-20714428
Length
33 bp
Sequences
Splice sites
3' ss Seq
TAATTTTTTTCCTTTTGCAGCCC
3' ss Score
10.43
5' ss Seq
CAGGTGAAA
5' ss Score
5.07
Exon sequences
Seq C1 exon
CATCTTGCAGTGACCCGTGAATACAATAATGAGTGTATGCGGAGGTACAGCTGGACACCTGACACCGTGGACCAATCACATAACACTGTGTGCAGTCCTGTGCACTCTGG
Seq A exon
CCCTTCCTCCCCGCTTCCTCAGTATGACTCCAG
Seq C2 exon
GTTCCTAGTGAGTTTTATGGTGGATGCTCGGGGCGGGTCGATGAGAGGAAGTCGTCACGATGGTATGCGCATAATCGTTCCACCCAGAAAGTGTCCAGCACCGACACGAATCACCTGTCGTTTAGCAAAGCGCCACCGCCTGGCATACCCACCACCTATGGTGGAGGGAGAGGGTCTTGTGAGCAGACTGGTGGAAATGGGGCCAGCTGGGGCACAGTTTTTAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000061736_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.270 A=0.167 C2=0.171
Domain overlap (PFAM):
C1:
PF0079115=ZU5=PU(1.0=2.7)
A:
PF0079115=ZU5=PU(1.0=8.3)
C2:
PF0079115=ZU5=FE(71.4=100)
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGACACCGTGGACCAATCAC
R:
TATGCGCATACCATCGTGACG
Band lengths:
125-158
Functional annotations
There are 2 annotated functions for this event
PMID: 22749401
Inclusion decreases interaction with ATP1A1 and CDH1, but not KCNQ2. By LUMIER.
PMID: 29398366
The axon initial segment (AIS), a subcellular structure important for action potential initiation, is diminished upon Rbfox depletion. An Rbfox-regulated splicing switch in ankyrin G (exclusion/inclusion of HsaEX0004144), the AIS interaction hub protein, regulates ankyrin G-beta spectrin affinity and AIS assembly.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]