RnoEX0009397 @ rn6
Exon Skipping
Gene
ENSRNOG00000053288 | Ank3
Description
ankyrin 3 [Source:RGD Symbol;Acc:620157]
Coordinates
chr20:20403689-20408649:+
Coord C1 exon
chr20:20403689-20403795
Coord A exon
chr20:20406040-20406072
Coord C2 exon
chr20:20408425-20408649
Length
33 bp
Sequences
Splice sites
3' ss Seq
CTTTCTAAATTATTTTGCAGCTA
3' ss Score
8.84
5' ss Seq
AAGGTGATA
5' ss Score
4.18
Exon sequences
Seq C1 exon
CACCTTCCATTCACGAGGGAGTTTGACTCCGACTCCCTCAGACACTACAGTTGGGCAGCAGACACACTGGACAATGTGAACCTGGTCTCCAGCCCTGTGCATTCTGG
Seq A exon
CTACTCGTCTCCGCTGCCTCAGTATGACTCAAG
Seq C2 exon
GTTTCTGGTTAGCTTTATGGTGGACGCGAGAGGGGGCTCCATGCGAGGAAGCCGCCACCACGGGATGCGGATCATCATCCCTCCGCGCAAGTGTACGGCCCCCACCCGCATCACTTGCCGCCTGGTAAAGAGACATAAACTGGCCAACCCACCCCCCATGGTGGAAGGAGAGGGATTAGCCAGTAGGCTGGTAGAAATGGGTCCTGCGGGGGCACAATTTTTAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000053288_MULTIEX4-3/3=2-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.042 A=0.000 C2=0.218
Domain overlap (PFAM):
C1:
PF0079115=ZU5=PU(1.0=2.8)
A:
PF0079115=ZU5=PU(1.0=8.3)
C2:
PF0079115=ZU5=FE(71.4=100)
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTGACTCCGACTCCCTCAGAC
R:
CCTCTCGCGTCCACCATAAAG
Band lengths:
118-151
Functional annotations
There are 2 annotated functions for this event
PMID: 22749401
Inclusion decreases interaction with ATP1A1 and CDH1, but not KCNQ2. By LUMIER.
PMID: 29398366
The axon initial segment (AIS), a subcellular structure important for action potential initiation, is diminished upon Rbfox depletion. An Rbfox-regulated splicing switch in ankyrin G (exclusion/inclusion of HsaEX0004144), the AIS interaction hub protein, regulates ankyrin G-beta spectrin affinity and AIS assembly.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]