DreEX0018219 @ danRer10
Exon Skipping
Gene
ENSDARG00000099045 | cacnb2a
Description
calcium channel, voltage-dependent, beta 2a [Source:ZFIN;Acc:ZDB-GENE-080808-7]
Coordinates
chr7:71611116-71617637:-
Coord C1 exon
chr7:71617579-71617637
Coord A exon
chr7:71611702-71611811
Coord C2 exon
chr7:71611116-71611267
Length
110 bp
Sequences
Splice sites
3' ss Seq
CTGTGTTTTCTGTGCTTCAGGAT
3' ss Score
10.8
5' ss Seq
TAGGTGAGA
5' ss Score
6.95
Exon sequences
Seq C1 exon
GTAACAGATATGATGCAGAAAGCGCTGTTTGACTTTCTGAAGCACCGGTTTGAGGGGAG
Seq A exon
GATAACCATCACAAGAGTGGCAGCTGATATCTCTCTGGCCAAACGCTCAGTACTGAACAACCCCAGTAAACACGCCATCATCGAGCGCTCAAACACACGCTCCAATCTAG
Seq C2 exon
CGGAGGTTCAGAGCGAGATCGAGAGGATATTTGAGCTGGCCCGAACGCTGCAGCTGGTGGTTTTGGACGCCGACACAATAAACCATCCGTCACAGCTGGGGAAAACCTGTCTGGCACCCATCATTGTCTACATCAAGATCACCTCTCCAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000099045-'12-16,'12-15,13-16
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.171 C2=0.000
Domain overlap (PFAM):
C1:
PF0062516=Guanylate_kin=FE(10.5=100)
A:
PF0062516=Guanylate_kin=FE(20.4=100)
C2:
PF0062516=Guanylate_kin=FE(27.6=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCAGAAAGCGCTGTTTGACTT
R:
TGGAGAGGTGATCTTGATGTAGACA
Band lengths:
194-304
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]