DreEX0021719 @ danRer10
Exon Skipping
Gene
ENSDARG00000101677 | chrnb4
Description
cholinergic receptor, nicotinic, beta 4 (neuronal) [Source:ZFIN;Acc:ZDB-GENE-120809-1]
Coordinates
chr18:50633372-50637781:+
Coord C1 exon
chr18:50633372-50633481
Coord A exon
chr18:50634021-50635035
Coord C2 exon
chr18:50637608-50637781
Length
1015 bp
Sequences
Splice sites
3' ss Seq
AAACTCTGGTGTCTCCACAGTGC
3' ss Score
8.27
5' ss Seq
AGCGTGAGT
5' ss Score
8.2
Exon sequences
Seq C1 exon
AACTGGAACGATTATCGCCTGTCGTGGGACCCGGCGGAGTACGAGGGCATCGACAAGCTGCGGATTCCCTCCAGACACATCTGGCTGCCTGATATAGTGCTGTACAACAA
Seq A exon
TGCTGACGGGACGTACGAGGTGACGGTGTTCACTAACGCCATCGTGCTCTTCAACGGCAGCATCGCGTGGCTCCCGCCGGCCATCTACAAGAGCGCCTGTAAGATCGAGGTCAAGCACTTTCCCTTCGACCAGCAGAACTGCACGCTCAAGTTCCGCTCCTGGACCTACGACCACACCGAGATCGACCTGCTGCTGAAGTCGGACGCCGCCAGCATGGACGACTTCACGCCCAGCGGCGAGTGGGACATCCTGGCGCTGCCAGGCCGTCGAACGGTCAGCCCGCTGGACGCCACGTACGTAGACCTGACCTACGACTTCATCATCAAGAGGAAGCCGCTGTTTTACACCATCAACCTGATCATCCCGTGTGTGCTCATCACCTCGCTGGCCATCCTGGTGTTCTACCTGCCGTCCGACTGCGGAGAGAAGATGACTCTGTGTATCTCCGTCCTACTAGCGCTCACCGTTTTCCTCTTGCTGATCTCCAAAATCGTGCCGCCCACTTCCCTGGACGTTCCTTTAATCGGGAAGTACCTGATGTTCACGATGGTTCTGGTGACCTTCTCGATTATCACTAGTGTGTGTGTGTTAAACGTGCACCATCGTTCTCCCAGTACCCACAGCATGCCGGCGTGGGTGAAGATCGTGTTTTTGGTGCGACTGCCGGCGCTGCTGTTTATGCAGAGACCGAGAGGACATTCGGCACGCCAGCGTCTGCGCCAGAACCGCCGTCTGCTTGGATTGGCTGATACTCCTGGCAGATCCGTCATGTGCTCGTCTGCGTCTGCGCTGCTGGCTGCGTCTTCGGCGTCCGCTCTTTCCTCTCCGGGACTCTTGTATAATAAAGGGCATTTCGGCTCTCGGAGAGCTGAAGGTCTGAATAATGGACACGGCTCCGCACAGGACCTGAAGGGGAACAGGCTGTATTGTGAATGGGGATCTGAGATTCAGGAGGCTGTGGACGGAGTTCGATATGTTGCTGATCACATGATGGGGAATGACGACGATCAAAGC
Seq C2 exon
GTCATCGAGGACTGGAAGTACGTGGCGATGGTGGTGGACCGGCTGTTCCTCTGGATCTTTGTGATCGTGTGTGTGGTGGGAACGCTGGGTCTTTTCCTGCAGCCGCTCTTCCAGAACCAGACCCTCGCCATCCAGCAGCCCAACACTGAGCCCGCCAGACGGGACGGCATGTGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000101677_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.015 C2=0.225
Domain overlap (PFAM):
C1:
PF0293118=Neur_chan_LBD=FE(18.0=100)
A:
PF0293118=Neur_chan_LBD=PD(54.4=33.0),PF103294=DUF2417=WD(100=43.4),PF0293211=Neur_chan_memb=PU(86.9=64.6)
C2:
PF0293211=Neur_chan_memb=PD(12.7=54.2)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATTATCGCCTGTCGTGGGAC
R:
TCACATGCCGTCCCGTCT
Band lengths:
275-1290
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]