RnoEX0021233 @ rn6
Exon Skipping
Gene
ENSRNOG00000014427 | Chrnb4
Description
cholinergic receptor nicotinic beta 4 subunit [Source:RGD Symbol;Acc:2351]
Coordinates
chr8:59609693-59617048:-
Coord C1 exon
chr8:59616939-59617048
Coord A exon
chr8:59615220-59616210
Coord C2 exon
chr8:59609693-59610629
Length
991 bp
Sequences
Splice sites
3' ss Seq
TATGTCCTGCCCTACCCCAGTGC
3' ss Score
7.18
5' ss Seq
AGTGTAAGT
5' ss Score
8.46
Exon sequences
Seq C1 exon
GAATGGACTGACTACCGCCTGGCCTGGAACAGCTCCTGCTATGAAGGGGTGAACATTCTGAGGATCCCCGCAAAGCGTGTCTGGTTGCCTGACATCGTGTTGTACAACAA
Seq A exon
TGCCGATGGCACCTATGAGGTGTCTGTCTACACCAACGTGATTGTGCGTTCCAATGGCAGCATCCAGTGGCTGCCCCCTGCTATCTACAAGAGTGCCTGCAAGATTGAGGTGAAGCACTTTCCCTTCGACCAGCAGAACTGCACCCTCAAATTCCGCTCCTGGACCTATGACCACACGGAGATTGACATGGTTCTTAAGTCGCCCACGGCCATCATGGATGACTTCACCCCCAGTGGTGAATGGGACATTGTGGCCCTCCCAGGACGGAGGACGGTGAACCCTCAGGACCCCAGCTACGTGGACGTGACCTATGACTTCATCATCAAGCGCAAGCCGCTCTTCTACACCATCAATCTTATCATTCCTTGTGTGCTCATCACCTCGCTGGCTATCCTGGTCTTCTACCTGCCCTCCGACTGTGGGGAGAAGATGACGCTCTGCATCTCTGTGCTGCTGGCACTCACGTTCTTCCTGCTGCTCATCTCCAAGATCGTGCCTCCCACCTCCCTTGACATACCGCTCATTGGCAAGTACCTCTTGTTCACCATGGTGCTGGTCACCTTTTCCATCGTCACCACTGTGTGTGTCCTCAATGTGCACCACCGCTCACCCAGCACTCACACCATGGCATCCTGGGTCAAGGAGTGCTTCCTGCACAAACTGCCCACCTTCCTCTTCATGAAGCGTCCCGGTCTTGAAGTCAGCCTGGTCAGGGTCCCTCATCCCAGCCAGCTGCACTTGGCCACAGCTGATACTGCAGCCACCTCTGCCTTAGGCCCCACCAGCCCATCCAACCTCTATGGGAGTTCCATGTACTTTGTGAACCCTGTCCCTGCCGCTCCTAAGTCTGCAGTCAGCTCCCACACAGCAGGCCTCCCCAGGGATGCCCGTCTGAGGTCCTCCGGGAGGTTCCGGGAAGATCTACAGGAAGCATTAGAGGGTGTCAGCTTCATCGCCCAGCATCTGGAGAGCGATGACCGAGATCAAAGT
Seq C2 exon
GTCATCGAGGACTGGAAGTTCGTCGCGATGGTTGTTGACCGCCTGTTCCTGTGGGTGTTCGTGTTTGTGTGTATTCTGGGCACCATGGGGCTCTTCCTGCCACCCCTTTTCCAGATCCACGCACCCTCCAAGGACTCCTAGGCTACCCGGCGTGTCTCGGGCCCCCGGGTTGTAGTGAGATGATATGAGAAGCCGTGTGGGAAGCTGACTGTTCCATTTGGGCCATAGCTAATGAAGCCCTAAGTAAAGCCATGACTATTGGCAATCAGCCCATCAAACTAGCCACAGCCACGAGAAGGACAGAGATAGATGCCTTCTGCTGATCCCTCTGATGCCGTCCAGTAAGTCCCTGAAGGAGGAGGATGAGGCTGCTGAATACCAGCCTGGCCTCCTTCCTTCCACGTGCCAGGCACCGGAGTCAGACAGCCGTGTATCATGCCGTCACACCCCAGCCCGCCTTCCCATCCATCTCCCTCTGATTCGCAGCTACCCACTAGATT
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000014427-'6-11,'6-10,9-11
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.003 C2=0.000
Domain overlap (PFAM):
C1:
PF0293118=Neur_chan_LBD=FE(17.5=100)
A:
PF0293118=Neur_chan_LBD=PD(54.4=33.8),PF0293211=Neur_chan_memb=PU(86.5=63.7)
C2:
PF0293211=Neur_chan_memb=PD(12.7=66.0)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAACATTCTGAGGATCCCCGC
R:
CGTGGCTGTGGCTAGTTTGAT
Band lengths:
353-1344
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]