DreEX0021727 @ danRer10
Exon Skipping
Gene
ENSDARG00000034307 | chrne
Description
cholinergic receptor, nicotinic, epsilon [Source:ZFIN;Acc:ZDB-GENE-040808-32]
Coordinates
chr5:37841136-37843318:-
Coord C1 exon
chr5:37843204-37843318
Coord A exon
chr5:37842360-37842564
Coord C2 exon
chr5:37841136-37841269
Length
205 bp
Sequences
Splice sites
3' ss Seq
ACCTCTCTTATATCCATTAGATT
3' ss Score
6.61
5' ss Seq
TTGGTGAGA
5' ss Score
6.29
Exon sequences
Seq C1 exon
GTATTTAATTTTCGTGATGTCTATGACCACACTCATTGTGACCAACTGCATTATTGTGCTCAACTACTCCCTCCGCAGTCCCAGCACACACAACATGTCCCAGAGCATCAAGCAT
Seq A exon
ATTTTCCTAGAAGTGGTCCCTCGTTATCTTGGCATGGCCCCCTTTGTGGATGAGGGGGACCAGGGTGGGGTGTATGAAATGAGGGAGAGGCGACGGAGTTCATTTGGGCTCATGCAGAGGGCTGAGGAATATGTTCTGAAACAGCCACGCAGTGAGATGATGTTTGATAAACAAAGAGAGAGACATGGACTCATGCGCACAATTG
Seq C2 exon
TGGATGAAATTGATGTGAGCAGTACCGCAAACCTCTACAAGAGTCTGGCAAAGACGGCCCCAGAAATAAAAGAGTGTGTGGATGCCTGTAACTTCATTGCTGAATCCACCAAGCAGCAGAATGACACTGGATCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000034307-'10-13,'10-12,12-13
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.014 C2=0.022
Domain overlap (PFAM):
C1:
PF0293211=Neur_chan_memb=FE(15.6=100)
A:
PF0293211=Neur_chan_memb=FE(28.0=100)
C2:
PF0293211=Neur_chan_memb=FE(18.1=100)
Main Skipping Isoform:
ENSDART00000136428fB1932
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TAATTTTCGTGATGTCTATGACCACA
R:
CGATCCAGTGTCATTCTGCTGC
Band lengths:
244-449
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]