DreEX0042422 @ danRer10
Exon Skipping
Gene
ENSDARG00000058543 | lama5
Description
laminin, alpha 5 [Source:ZFIN;Acc:ZDB-GENE-030131-9823]
Coordinates
chr23:9717837-9729030:-
Coord C1 exon
chr23:9728874-9729030
Coord A exon
chr23:9726572-9726710
Coord C2 exon
chr23:9717837-9717979
Length
139 bp
Sequences
Splice sites
3' ss Seq
GGCCGCTTTTTGCTCTCTAGCCT
3' ss Score
6.67
5' ss Seq
ACTGTAAGT
5' ss Score
8.59
Exon sequences
Seq C1 exon
ACCCCGGCCGGATCATTACTTCCCTGACTTGCACCATATGAAGTTTGAGGTGGAGGACGGCACCACCATGGATGGCCGTCCGGTGAGGTTTGGTTACAATCCACTGGAGTTTGAGAGCTTCAGCTGGAGGGGCTATGCTCAGATGTCCCCTATACAG
Seq A exon
CCTCGGGTTCTGGTGGAGGTGGTGGTTGGCTCTCCAGACCTGTTTCATGTGGTCTTGCACTATGTGAACCGCGGGGGTGTGGACGTGACGGGACAGGTCTCTGTGATCGAGGATGGCAGACATGTTCTGTGTGGGAACT
Seq C2 exon
GCTCTGAACAGTCCAAACAGATCGTCTTTGCCCCCAGTTCAGAACCAACATTTGTGAACGTGCCTCAAAACAGCTTTGTGGAGCCATTTGTCTTGAATCCGGGAACCTGGACTGTTATCATTGAGGCGGAAGGCATTTTGCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000058543-'24-23,'24-21,25-23
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.019 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0005319=Laminin_EGF=PD(28.3=24.5)
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAAGTTTGAGGTGGAGGACGG
R:
AAATGCCTTCCGCCTCAATGA
Band lengths:
256-395
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]