DreEX0045283 @ danRer10
Exon Skipping
Gene
ENSDARG00000059581 | mbd5
Description
methyl-CpG binding domain protein 5 [Source:ZFIN;Acc:ZDB-GENE-070209-125]
Coordinates
chr6:38686-45190:+
Coord C1 exon
chr6:38686-38788
Coord A exon
chr6:39272-39452
Coord C2 exon
chr6:44955-45190
Length
181 bp
Sequences
Splice sites
3' ss Seq
TGAAACGTCTGATTCCTCAGGTT
3' ss Score
7.66
5' ss Seq
CCAGTACGT
5' ss Score
7.19
Exon sequences
Seq C1 exon
CCCCAGCGGATCAGTGCTGTCCTGTCTCGAGCAGGTCAAGTCTTACCTGCTGACCGATGGCACCTGTAAATGTGGTCTGGAATGTCCACTCATCCTCCCGAAG
Seq A exon
GTTTTTAACTTTGACCCTGGAGCGGCGGTGAAGCAGCGGACAGCAGAGGACGTGAAGGCTGACGAGGACGTGACGAAGCTCTGCATCCACAAGCGGAAGCTGATCGCGGTGGCGGCGCTGCATAAGAGCATGGAGCTGCAGCCGCCGGCGCTCACGCTCACCAGCCCCGGAGGAGGCACCA
Seq C2 exon
GTGAGCCCAATCCGCTGAGCTGCAGCATGGCCAACACTGCTCATATGGGTGGCATGATGCCTGCCGCAGGACACATGTACCACCAACACCACCAGCAGCAGCTTCACCCGGCACTGCAGGGCATGCACGGAGTCTCTACATACCCGAGCCAGGGACACTCCTTTACTACGGGCCCTTTCACAGACAGCAGCGTCCCCAACTCCAGCAGCGTGCCCTGCCTGTACCAGGATTACCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000059581_MULTIEX1-1/5=C1-3
Average complexity
C2*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.311 C2=NA
Domain overlap (PFAM):
C1:
PF0142914=MBD=FE(47.9=100)
A:
PF0142914=MBD=PD(15.5=18.0)
C2:
NA
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGTCTCGAGCAGGTCAAGTCT
R:
AGGGCCCGTAGTAAAGGAGTG
Band lengths:
257-438
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]