DreEX0045845 @ danRer10
Exon Skipping
Gene
ENSDARG00000009681 | med27
Description
mediator complex subunit 27 [Source:ZFIN;Acc:ZDB-GENE-040426-1601]
Coordinates
chr8:11853311-11867774:-
Coord C1 exon
chr8:11867667-11867774
Coord A exon
chr8:11857866-11857907
Coord C2 exon
chr8:11853311-11853388
Length
42 bp
Sequences
Splice sites
3' ss Seq
TTCTCTTTTTTTCTTTTTAGCTG
3' ss Score
11.24
5' ss Seq
AAGGTGGGT
5' ss Score
8.23
Exon sequences
Seq C1 exon
GTGACTCTGGGGAAGGTTTTGAAAGCCATCGTGGTAATGAGGAGCCTTTTCATCGACCGCACGATTGTCAGAGGTTTCCACGAGAACATATACACAGAGGACCGAAAG
Seq A exon
CTGGACATCTGGTCGAAGTCCAACTATCAAGCATTTCAAAAG
Seq C2 exon
GTCACTGATCATGCAACCACAGCCCTCCTGCATTACCAGCTACCGCAGATGCCTGATGTTGTGGTTCGCTCCTTCATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000009681_MULTIEX1-3/4=2-4
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF115713=Med27=PU(3.4=8.3)
A:
PF115713=Med27=FE(14.9=100)
C2:
PF115713=Med27=FE(28.7=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGGAGCCTTTTCATCGACCG
R:
ACCACAACATCAGGCATCTGC
Band lengths:
135-177
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]