DreEX0050004 @ danRer10
Exon Skipping
Gene
ENSDARG00000032630 | neb
Description
nebulin [Source:ZFIN;Acc:ZDB-GENE-041111-216]
Coordinates
chr9:23000931-23007370:-
Coord C1 exon
chr9:23007266-23007370
Coord A exon
chr9:23003156-23003248
Coord C2 exon
chr9:23000931-23001038
Length
93 bp
Sequences
Splice sites
3' ss Seq
TTGCCTCTGTGTGTTTGAAGATT
3' ss Score
8.41
5' ss Seq
ACGGTACAC
5' ss Score
5.08
Exon sequences
Seq C1 exon
CTTCAGTACCAGATTGACCTTAAAAAGAGTAAGGGCAAAGTTTCAGCAGTACAGAACACCCCTGAAATGCTTCGTGTTAAAGAAAATACAAAGAATTTCAGCTCG
Seq A exon
ATTCATTACAAAGAAGATTTGGGAGGAGGAACGGCTATTTCAGAAACCCCAGAGATGGAGAGAGTTAAACGAAATCAGCAAAATATTAGCACG
Seq C2 exon
GTTTTGTACTCTGACAGCTTCCGTAAGCAGGTGCAAGGAAAGGCCGCCTTTGTGCTGGACACCCCCGAGATGAGACGCGTCAAAGAAACCCAGCGTATCATTTCTGGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032630_MULTIEX1-12/13=11-C2
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.350 A=0.544 C2=0.106
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(41.4=33.3),PF0088013=Nebulin=PU(55.2=44.4)
A:
PF0088013=Nebulin=PU(72.7=51.6)
C2:
PF0088013=Nebulin=PD(37.9=30.6),PF0088013=Nebulin=PU(55.2=44.4)
Main Skipping Isoform:
ENSDART00000142585fB4197
Other Inclusion Isoforms:
NA
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGGCAAAGTTTCAGCAGTACA
R:
CTGGGTTTCTTTGACGCGTCT
Band lengths:
167-260
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]