DreEX0050005 @ danRer10
Exon Skipping
Gene
ENSDARG00000032630 | neb
Description
nebulin [Source:ZFIN;Acc:ZDB-GENE-041111-216]
Coordinates
chr9:23000931-23007800:-
Coord C1 exon
chr9:23007696-23007800
Coord A exon
chr9:23001411-23001503
Coord C2 exon
chr9:23000931-23001038
Length
93 bp
Sequences
Splice sites
3' ss Seq
ACTGTTCTTCTCTACTCTAGGTG
3' ss Score
12.63
5' ss Seq
TCGGTATAC
5' ss Score
3.08
Exon sequences
Seq C1 exon
AAAAAATACAAGGAGGAGGCTGAGAAGACCATGTCTCACTATGTGCCTGTCCTGGACACCCCAGAAATGCAAAGAGTGCGTGAGAACCAGAAGAACTTCAGCACT
Seq A exon
GTGTTGTATAAGGATCTGTCGGCCAAGGGAACGCCTGTGGTGTTCACTCCAGAGATGGAGCGGGTCAAACGGAACCAATTGCACATTAGCTCG
Seq C2 exon
GTTTTGTACTCTGACAGCTTCCGTAAGCAGGTGCAAGGAAAGGCCGCCTTTGTGCTGGACACCCCCGAGATGAGACGCGTCAAAGAAACCCAGCGTATCATTTCTGGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032630_MULTIEX1-13/13=10-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.630 A=0.183 C2=0.106
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(41.4=33.3),PF0088013=Nebulin=PU(64.0=44.4)
A:
PF0088013=Nebulin=PD(18.2=12.9),PF0088013=Nebulin=PU(55.2=51.6)
C2:
PF0088013=Nebulin=PD(37.9=30.6),PF0088013=Nebulin=PU(55.2=44.4)
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGAGGAGGCTGAGAAGACCAT
R:
CTTTGACGCGTCTCATCTCGG
Band lengths:
179-272
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]