DreEX0060029 @ danRer10
Exon Skipping
Gene
ENSDARG00000010316 | qars
Description
glutaminyl-tRNA synthetase [Source:ZFIN;Acc:ZDB-GENE-040426-1011]
Coordinates
chr8:46372275-46377825:-
Coord C1 exon
chr8:46377675-46377825
Coord A exon
chr8:46374944-46375053
Coord C2 exon
chr8:46372275-46372350
Length
110 bp
Sequences
Splice sites
3' ss Seq
TTTATTTTATTTTTCCTCAGCGG
3' ss Score
8.55
5' ss Seq
GCAGTAAGA
5' ss Score
4.95
Exon sequences
Seq C1 exon
GCTCAAGGCCAGCTGGGGTCTTCTGCCATTGACAAGATCACTGGCACTCTGCTGTACAGTATGGTGACACGACTTAAAGACACGAACAGGCTTCCCTTTCTCACAAAATACATCATTTCACGAAAAATCACAACAGAACTGCAGCTGTCAG
Seq A exon
CGGCTTTGGACTTTGTGAAAAGCCACCCTCAGGGCGATGTGGATCAGCGCAAGTTTGAGGTGGCCTGTGGTGTGGGAGTGGTTGTCACACCTGAGCAGATAGAGGACGCA
Seq C2 exon
GTGGAATTGATTATCAGGAAACACAAAGATCAGCTGTTGGCAGAGAGATATCGCTTCAACATGGGGATACTCATGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000010316-'1-2,'1-1,2-2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0455810=tRNA_synt_1c_R1=FE(30.9=100)
A:
PF0455810=tRNA_synt_1c_R1=FE(22.2=100),PF0074916=tRNA-synt_1c=PU(7.3=21.6)
C2:
PF0455810=tRNA_synt_1c_R1=FE(15.4=100),PF0074916=tRNA-synt_1c=FE(22.9=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTCAAGGCCAGCTGGGGTC
R:
CCATGAGTATCCCCATGTTGAAGC
Band lengths:
226-336
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]