DreEX0064355 @ danRer10
Exon Skipping
Gene
ENSDARG00000018743 | scamp5a
Description
secretory carrier membrane protein 5a [Source:ZFIN;Acc:ZDB-GENE-030131-3188]
Coordinates
chr25:31426267-31441230:-
Coord C1 exon
chr25:31441129-31441230
Coord A exon
chr25:31428559-31428676
Coord C2 exon
chr25:31426267-31426924
Length
118 bp
Sequences
Splice sites
3' ss Seq
TGTTTCTGTTTCTCCTACAGTGG
3' ss Score
10.69
5' ss Seq
AAGGTACCA
5' ss Score
7.87
Exon sequences
Seq C1 exon
GTCAGACAGTTCTTTTAACTTCATGGCGTTCTTTTTCGTCTTCATGGCGCAAGTGGTGATTAGCATCATCCAGACGGTTGGAATCCCAGGATGGGGAGTTTG
Seq A exon
TGGCTGGTTGGCCACCATCACTTTTTTCAGCACTAATATCGGCTCTGCTGTTGTTATGCTGATTCCAACTATAATGTTCACTGCCGTGGCCGTGCTCTCCTTCATCGCCCTCACTAAG
Seq C2 exon
GTCCACAATTTCTACCGTGGCAGTGGGGGCAGCATGAGCAAGGCTCAGGAGGAGTGGACATCCGGTGCCTGGAAGAACCCTCACGTCCAGCAGGCGGCGCAGCAGGCGGCCATGGGGGCGGCACAGGGGGCCATGCAGGGCCAGCAGTACTCGGCCGCACCGACCTACAACTACGACGATCCAATGTAGACCCTCTCCATTCACAACAGAGGGAGGGAACAAAAACAGGGTTTCTGGGAGGGAATTTCTAAGGGGGAAAAGCTGCGGACTGGTTTAAAACAGGGGAGGGGAAAGCAAAGATATGGCCAAATTAGAATTTTCGCAACTACAACAATACAGAAGCTAAATGTGTTGATAGTTTAAGGAGTTGCGTTTTTAGAGCCCCTCAATAGGAACGTCACTCTCGCCATGTGGTATTACAGTTAACTAATGGTATGTTGTGTTTCTCCACTTGTCGTATTGTAAACATGACTAAACTAGAGCACCAATAAGGAAACATT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000018743_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF041448=SCAMP=FE(19.3=100)
A:
PF041448=SCAMP=FE(22.2=100)
C2:
PF041448=SCAMP=PD(4.5=12.7)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGACGGTTGGAATCCCAGGAT
R:
TGTTCCCTCCCTCTGTTGTGA
Band lengths:
252-370
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]