DreEX6000523 @ danRer10
Exon Skipping
Gene
ENSDARG00000063578 | DGKI
Description
diacylglycerol kinase, iota [Source:HGNC Symbol;Acc:HGNC:2855]
Coordinates
chr4:4622661-4627371:-
Coord C1 exon
chr4:4627260-4627371
Coord A exon
chr4:4626714-4626913
Coord C2 exon
chr4:4622661-4622723
Length
200 bp
Sequences
Splice sites
3' ss Seq
TTCTTTCCATCCTCCACCAGGCG
3' ss Score
9.94
5' ss Seq
TGAGTGAGT
5' ss Score
6.51
Exon sequences
Seq C1 exon
ATACTGTGCTGGAACAATGCCATGGGGAAACACAGGAGACCACCGAGACTTTGAGCCACAGCGACATGATGATGGCTGCATCGAGGTCATCGGCTTCACCATGGCCTCTCTG
Seq A exon
GCGGCGCTGCAGGTTGGTGGTCATGGGGATCGTCTCCATCAGTGTCGAGAGGTGCTCATCACCACCTTCAAGACTCTGCCAGTGCAGGTAGATGGAGAGCCGTGCCGTCTGGCGCCCTCCACCCTGCGCATCTCTCTGAGGAACCAGGCCAACATGGTGCAGAAGAGCAAGAGACGCACATCTGTACCGCTGCTCAATGA
Seq C2 exon
CATTCAGAAAGTGTGTGCAGCCGACCTGCGCCGCCTCTCTGCTCCCCCAGACTCCTTCTCTGT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000063578-'22-27,'22-25,23-27=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.158 A=0.015 C2=0.045
Domain overlap (PFAM):
C1:
PF0060914=DAGK_acc=FE(23.4=100)
A:
PF0060914=DAGK_acc=PD(20.9=49.3)
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
ACTGTGCTGGAACAATGCCAT
R:
CAGAGAAGGAGTCTGGGGGAG
Band lengths:
172-372
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]