BtaEX6028848 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000019980 | DGKI
Description
diacylglycerol kinase, iota [Source:HGNC Symbol;Acc:HGNC:2855]
Coordinates
chr4:101995813-102057381:-
Coord C1 exon
chr4:102057270-102057381
Coord A exon
chr4:102030342-102030541
Coord C2 exon
chr4:101995813-101995913
Length
200 bp
Sequences
Splice sites
3' ss Seq
TTTGCCTCTTGTCTTTACAGGCT
3' ss Score
11.65
5' ss Seq
TGAGTGAGT
5' ss Score
6.51
Exon sequences
Seq C1 exon
ATATTGTGCTGGCACAATGCCCTGGGGGAACCCAGGAGATCACCATGACTTTGAACCTCAGCGTCATGATGATGGTTATATTGAAGTCATTGGGTTCACCATGGCATCTTTG
Seq A exon
GCTGCCCTGCAAGTCGGAGGCCATGGAGAAAGGCTGCACCAGTGTCGGGAGGTCATGCTGCTAACTTACAAATCCATCCCCATGCAAGTGGATGGGGAGCCCTGCAGGTTGGCCCCGGCTATGATTCGGATCTCACTGAGGAATCAGGCCAACATGGTGCAGAAGAGCAAGAGGAGAACGTCCATGCCTTTACTCAATGA
Seq C2 exon
TCCCCAGTCTGTTCCAGACCGTCTGAGGATCCGGGTGAACAAAATCAGTTTACAAGACTATGAAGGATTCCACTACGACAAAGAGAAACTCCGGGAAGCCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000019980-'26-27,'26-25,27-27=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.224 A=0.119 C2=0.029
Domain overlap (PFAM):
C1:
PF0060914=DAGK_acc=FE(23.4=100)
A:
PF0060914=DAGK_acc=PD(20.9=49.3)
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
ACCCAGGAGATCACCATGACT
R:
AGGCTTCCCGGAGTTTCTCTT
Band lengths:
184-384
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]