DreEX6000990 @ danRer10
Exon Skipping
Gene
ENSDARG00000063097 | scube1
Description
signal peptide, CUB domain, EGF-like 1 [Source:ZFIN;Acc:ZDB-GENE-050302-78]
Coordinates
chr4:8907332-8909383:-
Coord C1 exon
chr4:8909222-8909383
Coord A exon
chr4:8908864-8909061
Coord C2 exon
chr4:8907332-8907484
Length
198 bp
Sequences
Splice sites
3' ss Seq
ATGCCGCTGGTCTATTCCAGATC
3' ss Score
8.64
5' ss Seq
GTGGTATGG
5' ss Score
5.61
Exon sequences
Seq C1 exon
TTCACTGTGCTCTTGGCCATTATTATAATGCCACCACACATCGGTGCATCCGCTGTCCAGCTGGTACCTATCAGTCTGAATCCGGACAGAACTACTGCATCACCTGCCCTGGAAACACCACTACAGATTTCGACGGCGCTAACAACGTGTCTCACTGCAAAA
Seq A exon
ATCAGATATGTGGTGGAGAGCTGGGCGACTTCATGGGCTACATCGAATCTCCGAACTATCCCGGTGACTATCCATCTAATGTGGACTGCGTCTGGACCATTAACCCTCCAAACAAAAGGCGGATCCTTATTGTTGTACCAGAGATCTTCCTCCCCATCGAGGATGAGTGCGGGGATGTTCTCGTCATGAGGAAGAGTG
Seq C2 exon
CCCTGCCCACTTCCATTACAACTTATGAGACCTGCCAAACTTATGAGCGACCGATCGCCTTCACCTCCCGTTCACGCAAACTGTGGATCCAGTTTAAATCCAATGAGGGTAACAGTGGAAAGGGATTTCAGGTTCCATATGTCACTTATGATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000063097-'20-25,'20-24,21-25=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF076998=GCC2_GCC3=WD(100=87.3)
A:
PF0043115=CUB=PU(56.4=92.5)
C2:
PF0043115=CUB=PD(42.7=90.4)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTCACTGTGCTCTTGGCCATT
R:
CTGGATCCACAGTTTGCGTGA
Band lengths:
254-452
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]