DreEX6002509 @ danRer10
Exon Skipping
Gene
ENSDARG00000026855 | cacna2d4a
Description
calcium channel, voltage-dependent, alpha 2/delta subunit 4a [Source:ZFIN;Acc:ZDB-GENE-041210-213]
Coordinates
chr4:20558989-20568789:-
Coord C1 exon
chr4:20568729-20568789
Coord A exon
chr4:20559359-20559509
Coord C2 exon
chr4:20558989-20559063
Length
151 bp
Sequences
Splice sites
3' ss Seq
TTTTTTTTTTTCATGCTCAGGTA
3' ss Score
10.19
5' ss Seq
GCGGTAAGA
5' ss Score
9.63
Exon sequences
Seq C1 exon
GTATTAAGTGGACACCTGATGAAAATGGAGTTGTCACTTTTGATGGAAGGAATCGCAACTG
Seq A exon
GTACATTCAAGCAGCCACATCTCCTAAAGACATCATTATCGCTGTGGACATCAGCGGGAGTATGAAGGGTTTGAGACTCACCATCGCAAAACACACCATCAACACCATTCTAGACACACTAGGCGAAAACGACTTTGTCAACGTCATTGCG
Seq C2 exon
TACAGTGATTATGTCCAATATGTAGAGCCCTGTTTTAAAGGCACACTGGTACAAGCTGATCTGGATAACCGTGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000026855-'7-7,'7-6,8-7=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF083996=VWA_N=PD(3.4=19.0)
A:
PF137681=VWA_3=PU(24.7=80.4)
C2:
PF137681=VWA_3=FE(15.1=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGTGGACACCTGATGAAAATGGA
R:
CTCACGGTTATCCAGATCAGCT
Band lengths:
130-281
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]