DreEX6002514 @ danRer10
Exon Skipping
Gene
ENSDARG00000026855 | cacna2d4a
Description
calcium channel, voltage-dependent, alpha 2/delta subunit 4a [Source:ZFIN;Acc:ZDB-GENE-041210-213]
Coordinates
chr4:20555329-20558526:-
Coord C1 exon
chr4:20558448-20558526
Coord A exon
chr4:20556655-20556788
Coord C2 exon
chr4:20555329-20555391
Length
134 bp
Sequences
Splice sites
3' ss Seq
TCACTGATGTTTGTAATTAGGTT
3' ss Score
8.3
5' ss Seq
GTGGTGAGC
5' ss Score
6.64
Exon sequences
Seq C1 exon
GTGCGCGTGTTCACGTATCTGATTGGACGAGAGCTTACATTTGCCAGCAATGTCAAATGGATTGCTTGCAATAATAAAG
Seq A exon
GTTACTACACTCACATCTCTACACTGGCGGATGTACAGGAGAATGTGATGGAATATCTTCATGTGCTGAGCAGACCAATGGTCATCAACCATGATCATGACATCATTTGGACAGAGGCTTACATGGACAGTGTG
Seq C2 exon
GCCGAGAGTTTGCTGTTGATGACCTCTGTAGCCATGCCTGTGTTCAGCAAGAAAGAAGAAACA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000026855-'12-14,'12-11,13-14=AN
Average complexity
A_C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF137681=VWA_3=FE(15.7=100)
A:
PF137681=VWA_3=PD(3.0=10.9),PF0274313=Cache_1=PU(9.0=17.4)
C2:
PF0274313=Cache_1=FE(23.6=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGCGCGTGTTCACGTATCT
R:
TCTTCTTTCTTGCTGAACACAGGC
Band lengths:
138-272
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]