DreEX6005727 @ danRer10
Exon Skipping
Gene
ENSDARG00000076104 | SEMA4B (2 of 2)
Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B [Source:HGNC Symbol;Acc:HGNC:10730]
Coordinates
chr7:29981210-29983601:+
Coord C1 exon
chr7:29981210-29981272
Coord A exon
chr7:29981367-29981465
Coord C2 exon
chr7:29983490-29983601
Length
99 bp
Sequences
Splice sites
3' ss Seq
TTGATTCTTATGTGTTTCAGACT
3' ss Score
7.71
5' ss Seq
ATTGTGAGT
5' ss Score
7.35
Exon sequences
Seq C1 exon
CTCACGTGGAGCACTCCTGAAACAAAGAGGGGAGAGTGTATCTTTAAGGGAAAAGACCCCCAG
Seq A exon
ACTGATTGTTTCAACTACATCAAGATCTTACTGCGTCTGAACAGCACACACCTGTTTGTGTGTGGAACGTATGCATTCAGCCCCATCTGCACTTATATT
Seq C2 exon
AACATTTCAAGTTTTTCTCTGGAAGCAGATGAAAAGGGTGAACCTATTATGGAGGACGGCCGTGGACGCTGTCCATTTAATCCAGAATACAGATCTACAGCTATTACAGTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000076104-'3-7,'3-5,4-7=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.125
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(17.1=100)
A:
PF0140314=Sema=FE(27.4=100)
C2:
PF0140314=Sema=PD(5.1=54.5)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGCACTCCTGAAACAAAGAGGG
R:
CCACTGTAATAGCTGTAGATCTGT
Band lengths:
167-266
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]