DreEX6007146 @ danRer10
Exon Skipping
Gene
ENSDARG00000015349 | mfge8a
Description
milk fat globule-EGF factor 8 protein a [Source:ZFIN;Acc:ZDB-GENE-050327-9]
Coordinates
chr7:48517679-48520557:-
Coord C1 exon
chr7:48520402-48520557
Coord A exon
chr7:48517963-48518107
Coord C2 exon
chr7:48517679-48517866
Length
145 bp
Sequences
Splice sites
3' ss Seq
TTATTATTACTTTTTTTTAGGTA
3' ss Score
10.86
5' ss Seq
ATGGTAATT
5' ss Score
6.49
Exon sequences
Seq C1 exon
ATAGACATGCAAAAGAAGATGCGTCTCACAGGAATCATCACTCAAGGTGCCAGTCGCATGGGGACAGCTGAGTTTATCAAGGCTTTCAAGGTGGCATCCAGTTTTGATGGCAAAACCTACACTGTGTACAGAGCAGATGGCCAGAGAAAAGACAGA
Seq A exon
GTATTTGCAGGAAATTCAGATAACGATGGTACCAAGACAAACATGTTTGACCCTCCCATTGTGGCCCAGTTTATTCGCATCATTCCTGTTGTCTGCCGTAAAGCCTGTACTTTGCGGATGGAGCTTGTGGGCTGTGAGCTCAATG
Seq C2 exon
TCGGCTGCACTGATCCACTGGGCATGAAATCCCGACTCATCTCTGATGTTCAGCTCACAGCATCTAGTACTTTCCGCACCTGGGGTATAGATGCTTTTACCTGGTACCCTTTCTACGCTCGTCTGGACAAGCAAGGCAAAACTAATGCATGGTCAGCAGCTAACAACAACCGCTCTGAGTGGCTCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000015349-'7-10,'7-7,9-10=AN
Average complexity
A_C3
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.143 C2=0.015
Domain overlap (PFAM):
C1:
PF0075420=F5_F8_type_C=FE(36.7=100)
A:
PF0075420=F5_F8_type_C=PD(29.5=83.7)
C2:
PF0075420=F5_F8_type_C=PU(32.1=66.2)
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTTTCAAGGTGGCATCCAGT
R:
CCACTCAGAGCGGTTGTTGTT
Band lengths:
257-402
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]