DreEX6011376 @ danRer10
Exon Skipping
Gene
ENSDARG00000035507 | ddx31
Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 [Source:ZFIN;Acc:ZDB-GENE-030131-3973]
Coordinates
chr5:29126275-29127099:+
Coord C1 exon
chr5:29126275-29126325
Coord A exon
chr5:29126464-29126603
Coord C2 exon
chr5:29127015-29127099
Length
140 bp
Sequences
Splice sites
3' ss Seq
GTGTCATGTTTCTGTTTTAGTAC
3' ss Score
10.02
5' ss Seq
CAGGTGTGT
5' ss Score
6.99
Exon sequences
Seq C1 exon
GATGTAGCTGCACGTGGTTTGGATTTGCCTCAGGTTACTTGGATTGTTCAG
Seq A exon
TACAATCCTCCAGTTTCTGCAGCAGAATATGTTCATCGTGTGGGCCGCACAGCTCGAATCGGAGCTCAGGGTAGCAGCCTGCTCTTTCTCACCCCCTCCGAGACCGCTTTTGTGGATGTGCTGGCCAATCACAACATCAG
Seq C2 exon
TTTGTCTGAGATGAAGATGGTGGACATCCTGTCCACACTAATGAAAGACGAGCGCTTTAAAGGCAGAGGGAAATGGGACAGCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000035507-'16-16,'16-15,17-16=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0027126=Helicase_C=PU(42.1=94.1)
A:
PF0027126=Helicase_C=PD(52.6=42.6),PF047579=Pex2_Pex12=PU(2.6=8.5)
C2:
PF047579=Pex2_Pex12=FE(18.2=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGTAGCTGCACGTGGTTTGG
R:
TGTCCCATTTCCCTCTGCCTT
Band lengths:
130-270
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]