DreEX6011633 @ danRer10
Exon Skipping
Gene
ENSDARG00000079585 | pkn3
Description
protein kinase N3 [Source:ZFIN;Acc:ZDB-GENE-030131-1902]
Coordinates
chr5:31243080-31247873:-
Coord C1 exon
chr5:31247722-31247873
Coord A exon
chr5:31245262-31245478
Coord C2 exon
chr5:31243080-31243262
Length
217 bp
Sequences
Splice sites
3' ss Seq
TTTTTCTTTTCATTGTCCAGGCT
3' ss Score
8.64
5' ss Seq
CAGGTACTG
5' ss Score
9.04
Exon sequences
Seq C1 exon
GTCGCCCTTCTGAAACAATCAGTCCTCTCGAGCTGCGGGTGGAGGAGCTCAGGCACCATCTAAGGATTGAGGCAGCTGTGGCAGAAGGTGCCAAAAATGTGGTTAAGCAGCTTGGTGTCCGTAAAGTGCAAGACCGACGTGCTCTAGCGGAA
Seq A exon
GCTCAGGCCCGTTTGCAGGAATCCTCTCAAAAACTGGACCTGTTGCGTTTGTCTCTGGAGCAGCAGCTTGGAGAACTTCCCCAAGACCATCCCAAACGAGCTGCCATTAAAGAAGAGCTGACAGTAGGTTCCTCTCCTGGTATTGGGTTGCAAAGGGAACGCCTGCGCTCTTCATCTTCAGCATCCTCTTCTCTCTTTAAACCAGCCAGCCTGACAG
Seq C2 exon
GGCGATTGGAGGTCAGGTTGATGGGTTGTCAGGACCTGCTGGAGTCAGTTCCAGGCCGCTGTCGTGTAAGCAACATGTCCTCCGCTCCTGGAAGTCCATCTGACTCCAAATCACTAAGAATGAGAACCGGCCTTTCCACTCGCAGCACCAACGGCAAGACCACCAAGACTGATGAACTGTCAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079585-'9-8,'9-7,11-8=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.255 A=0.548 C2=0.613
Domain overlap (PFAM):
C1:
PF0218511=HR1=PU(53.4=76.5)
A:
PF0218511=HR1=PD(43.8=43.8)
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAATCAGTCCTCTCGAGCTGC
R:
GTCTTGGTGGTCTTGCCGTTG
Band lengths:
306-523
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]