DreEX6013872 @ danRer10
Exon Skipping
Gene
ENSDARG00000029372 | rasa4
Description
RAS p21 protein activator 4 [Source:ZFIN;Acc:ZDB-GENE-060526-349]
Coordinates
chr5:58708551-58711185:+
Coord C1 exon
chr5:58708551-58708723
Coord A exon
chr5:58710750-58710887
Coord C2 exon
chr5:58711050-58711185
Length
138 bp
Sequences
Splice sites
3' ss Seq
TTTGGTCTTTGACTCTATAGAAG
3' ss Score
6.16
5' ss Seq
AAAGTTAGT
5' ss Score
4.24
Exon sequences
Seq C1 exon
ATCTTGAACTTCAGACACGAATGAGCCTCCAGTGCGGCACTATGGAGAAAGAGGGTTTTCTCTTCCTGCACAGGACCAAGGACAAGTGTATGCCAATGACTTCTCCCTTTAAGAAGTACTATGTGACGCTCAGTAAGGACACGCTGTCTTATTCCAGGACTCTGCACTCTAAG
Seq A exon
AAGAGCTCATCTATTTCCCTGCCAAAGATCCGTGCGGTAGAGAAGGTGGAGGAGAAGTGTTTCGGCAGTGCCAACGTCATGCAGATCATCTCCAGTGAAGACTCAGGCCAGCAGGAGACCCTATACCTCGACTGCAAA
Seq C2 exon
AGTGTAAATGAGTTGAATCACTGGCTGTCGGCTCTCAGAAAAGCCTGCAGTCACAACACAAACACTATGAGCTGCTTTCATCCCGGCATCTACAAAGCAGACAGATGGAGCTGCTGCCATCAGAAGGAGAAATCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000029372-'17-19,'17-18,18-19=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0016924=PH=PU(40.2=74.1)
A:
PF0016924=PH=FE(42.1=100)
C2:
PF0016924=PH=PD(15.0=34.8),PF0077914=BTK=PU(68.8=47.8)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCGGCACTATGGAGAAAGAGG
R:
GCAGCTCCATCTGTCTGCTTT
Band lengths:
254-392
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]