Special

MmuEX0038884 @ mm10

Exon Skipping

Gene
ENSMUSG00000004952 | Rasa4
Description
RAS p21 protein activator 4 [Source:MGI Symbol;Acc:MGI:1858600]
Coordinates
chr5:136103837-136105739:+
Coord C1 exon
chr5:136103837-136104006
Coord A exon
chr5:136104516-136104653
Coord C2 exon
chr5:136105604-136105739
Length
138 bp
Sequences
Splice sites
3' ss Seq
TTTTTTTCTGGCTGCCCCAGAAA
3' ss Score
7.85
5' ss Seq
AAGGTGTGG
5' ss Score
5.28
Exon sequences
Seq C1 exon
AGCTGGACCTGCAGCGGGCTTTGAACTCACAGGCACCACCTGTGAAGGAGGGACCATTGTTCATCCACAGGACCAAAGGCAAAGGCCCTCTTGCATCCTCCTCGTTCAAGAAACTCTACTTCTCCCTTACCACTGAAGCCCTCAGCTTCGCCAAGACGTCCAGCTCCAAG
Seq A exon
AAAAGCACCTTCATCAAGCTGGCCAGCATCCGGGCTGCGGAGAAGGTGGAGGAAAAGAGCTTCGGCAGCTCCCACATCATGCAGGTCATCTATGCAGATGATGTTGGCCGTGCCCAGACTGTCTACCTGCAGTGCAAG
Seq C2 exon
TGTGTGAACGAGTTGAACCAGTGGCTGTCTGCATTGCGCAAAGCGAGCACCAACAACAGGGGCCTCCTACGGTCATACCATCCTGGCATCTTCCGTGGGGACAAGTGGAGCTGCTGTCACCAGAAGGACAAGACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000004952-'36-44,'36-38,38-44
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
Alternative protein isoforms (Ref)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.082 A=0.000 C2=0.002
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0016924=PH=PU(40.2=75.4)

							
A:
PF0016924=PH=FE(42.1=100)

							
C2:
PF0016924=PH=PD(15.0=34.8),PF0077914=BTK=PU(68.8=47.8)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000037869





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000098136, ENSMUSP00000118929
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm9




Conservation


Human
(hg38)
chr7:102490986-102491123 
ALTERNATIVE
HsaEX0052378
(RASA4B)
Human
(hg19)
chr7:102131433-102131570 
ALTERNATIVE
HsaEX0052378
(RASA4B)
Rat
(rn6)
chr12:23604069-23604206 
HIGH PSI
RnoEX0074207
(Rasa4)
Cow
(bosTau6)
chr25:35080911-35081048 
HIGH PSI
BtaEX6110396
([1])
Chicken
(galGal4)
chr19:4169668-4169805 
HIGH PSI
GgaEX7009930
([2])
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
chr5:58710750-58710887 
HIGH PSI
DreEX6013872
(rasa4)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
TTTGAACTCACAGGCACCACC

				
R: 
ACGGAAGATGCCAGGATGGTA

				
Band lengths: 
247-385

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types





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