DreEX6016352 @ danRer10
Exon Skipping
Gene
ENSDARG00000079912 | rapgefl1
Description
Rap guanine nucleotide exchange factor (GEF)-like 1 [Source:ZFIN;Acc:ZDB-GENE-131121-404]
Coordinates
chr3:23204220-23212450:-
Coord C1 exon
chr3:23212386-23212450
Coord A exon
chr3:23210316-23210370
Coord C2 exon
chr3:23204220-23204543
Length
55 bp
Sequences
Splice sites
3' ss Seq
CTTTCTTTCTTTCTTTCCAGCAC
3' ss Score
12.25
5' ss Seq
TAGGTATGT
5' ss Score
7.76
Exon sequences
Seq C1 exon
ATCTGACTTTCCTTCATGAGAGCTGTAAGACTTTCCATGGAGAGCTGGTTAATTTTGAGAAGATG
Seq A exon
CACAAAGTGGCGGAGATGGTGCGCAGCATCAGGCGGTATCGGAGCTCTCAGTTAG
Seq C2 exon
CTATGGAGGCCGAGCCGTCCCCTTCGCACCTCCAGACGAAAGCGTACGTCCGGCAGCTGCAGGTCATCGACAATCAGAACCTGCTTTTTGAGATGTCCAGCAAACTGGAGCCAAAAGACATGTGAAGGGACCTACAAGCCATATCTAGAAAACCTCCAGACACAGACACTTCATTCTCCACCGTCACGGATCAAGCCAGCTGGGCTGTCAATCATCTCAGGCTCCGCCTCCGCATCACCGATCGACGAATAGTGTCCATGAATTGTGCTTTGAGTGAGATTCTGTGTCAGTGTTGATTATGAGCATTCATTATTCGCCATATCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079912-'12-18,'12-17,13-18=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref, Alt. Stop)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.027 C2=0.122
Domain overlap (PFAM):
C1:
PF0061714=RasGEF=FE(11.4=100)
A:
PF0061714=RasGEF=PD(0.5=5.3)
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGACTTTCCATGGAGAGCTGGT
R:
GCTGGACATCTCAAAAAGCAGG
Band lengths:
139-194
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]