DreEX6028687 @ danRer10
Exon Skipping
Gene
ENSDARG00000017004 | myo10
Description
myosin X [Source:ZFIN;Acc:ZDB-GENE-070912-277]
Coordinates
chr2:42778937-42779508:-
Coord C1 exon
chr2:42779402-42779508
Coord A exon
chr2:42779085-42779309
Coord C2 exon
chr2:42778937-42778979
Length
225 bp
Sequences
Splice sites
3' ss Seq
TTGCTCTCTGCTGGTCACAGGAG
3' ss Score
6.17
5' ss Seq
GAGGTGGGC
5' ss Score
6.32
Exon sequences
Seq C1 exon
GTGTTTATGAGAGAGAGTTTGGAGCTGCGGTTAGAAAAACAAAGAGAAACAGAAGTTCTCAAAGCAGCATCAATTATCCAAGCTCATATACTGGGCTACAGAGCACG
Seq A exon
GAGGCAGTACAGACGCCTGTTGCTGTGCATTGTTGTCATTCAGAAAAATTACCGTGCTCTATATTGGCGCCGACGCTTTCAGACAATGCGATGGGCCACCGTCACCTTACAGAAGAGGCTCAGAGGTCAAAGGGCACGAAGGCTCTATGTTCACCTTTTAGAGGAGAAGAGGAAGAGGGAAGAGGAAGAAAGGAGACACAGAGAAGAACAGGAGGCACTGGAGAG
Seq C2 exon
AGAGAGACATCGCTTGTTGGAGTTGGAGCTTTTAGCACAAGCA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000017004-'27-22,'27-21,28-22=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.171 C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=PD(0.5=8.3),PF0061222=IQ=PU(76.2=44.4)
A:
PF0061222=IQ=PD(19.0=5.3)
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGTTTATGAGAGAGAGTTTGGAGCT
R:
TGCTTGTGCTAAAAGCTCCAAC
Band lengths:
150-375
Functional annotations
There are 2 annotated functions for this event
PMID: 20731332
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: comigration in gel electrophoresis, competition binding. ELM ID: ELMI001883; ELM sequence: LYCVVIIQKNYRAFLLRRRF; Overlap: FULL
PMID: 20731332
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: comigration in gel electrophoresis, competition binding. ELM ID: ELMI001884; ELM sequence: KKAAIVFQKQLRGQIARRVY; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]