DreEX6029964 @ danRer10
Exon Skipping
Gene
ENSDARG00000006526 | fn1b
Description
fibronectin 1b [Source:ZFIN;Acc:ZDB-GENE-030131-6545]
Coordinates
chr1:4811283-4812240:-
Coord C1 exon
chr1:4811753-4812240
Coord A exon
chr1:4811527-4811665
Coord C2 exon
chr1:4811283-4811444
Length
139 bp
Sequences
Splice sites
3' ss Seq
CACAATTGCTTGTTTTGCAGATG
3' ss Score
8.85
5' ss Seq
CAGGTAAAA
5' ss Score
8.59
Exon sequences
Seq C1 exon
AGCTGATATCCGGTCTACCTAGCCTGCCCAGTCATGGTGGCCGAGACAAGCTGGATGTGCCAGAGCCTGACAACAGAATTAATACAGTGGGTCCTACTGGTCATGATTCAACGGATGCGCACGGGCAGCACGTGGAGTACACCGAATACAATAATCAGCCCAAACAACCCAACATAGGTTACCAGCCTCAAAATCGTCAGCCAATCTCTCAACCTAACCAACCTCGACCACAGCCTTATGTGCCTCAAGTTGGAGAGACCCTAGTCTATGTCCCCCAGGTGGGACCCGATGGGGGTCGTGTGCCTCAAATTATTCAGGTTAGTGAGAGGCCTGGCGATGGCCATACTTTTGGTTTTCCAGAGGACAGGACAGGAAGGCCGCAGGAAGCTCAGACCCAGACTACCATCTCATGGCAGCCCTTCCAGCAGAGTTCTGCCTACCTGGTATCATGTCAGCCAATCACCCATCCAGATGAGAAGATGTTCCAG
Seq A exon
ATGCGTGTCCCAGGAACATCCACAACTGCCACTCTTATTGGTCTCACCTCTGGTGCGTCCTATAATGTCATTGTGGAGGCCTTGAAAGGAGCCCTCAAGCACAAAATCCTAGAGGAAGTCGTCACTGCTGGAAATACAG
Seq C2 exon
TTCCAGGAGATGCTACATCCAGCAAGGACTCGTGTTATGACACTTTCACGGCCACTCACCACGATGTCGGAGCGGAATGGGAGCGCATGTCTGATACAGGCTTTAAACTCTGGTGTAGATGTCTTGGATTGGGAAGTGGACATTTCAGATGTGACTCTTCCA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000006526-'52-56,'52-55,53-56=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.871 A=0.213 C2=0.036
Domain overlap (PFAM):
C1:
PF0004116=fn3=PU(54.4=26.4)
A:
PF0004116=fn3=PD(43.0=72.3)
C2:
PF0003913=fn1=WD(100=72.7)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGGGTCGTGTGCCTCAAATTA
R:
GAGTGGCCGTGAAAGTGTCAT
Band lengths:
254-393
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]