DreEX6032870 @ danRer10
Exon Skipping
Gene
ENSDARG00000060184 | pkn1a
Description
protein kinase N1a [Source:ZFIN;Acc:ZDB-GENE-061207-17]
Coordinates
chr1:45136645-45137157:+
Coord C1 exon
chr1:45136645-45136720
Coord A exon
chr1:45136799-45136890
Coord C2 exon
chr1:45136981-45137157
Length
92 bp
Sequences
Splice sites
3' ss Seq
GTCATATCTGTGTCTATCAGGTG
3' ss Score
8.47
5' ss Seq
AAGGTAAAC
5' ss Score
8.14
Exon sequences
Seq C1 exon
TAAACAGAAGAAGACTTCTCTGAGCCTGCAGGATTTCCGACTAATTGCAGTTCTTGGCAGAGGCCATTTTGGAAAG
Seq A exon
GTGCTGCTGGCGGAATATAAGAAGTCAGGGAGTATGTATGCCATTAAAGCTCTAAAGAAAGGAGACATTGTAGCCAGAGATGAAGTAGAAAG
Seq C2 exon
TTTGATGTGTGAGAAGCGTATTTTCGAGGCAGTGAACGGTGCACAACACCCCTTCCTTGTCAACCTGTTTGCGTGTTTCCAGACTCCAGAACATGTGTGTTTTGTGATGGAGTACACAGCAGGCGGTGACCTCATGATGCACATACATGCAGACGTCTTCACCGAGCCACGGGCTGT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000060184-'17-18,'17-17,18-18=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.077 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0006920=Pkinase=PU(5.0=50.0)
A:
PF0006920=Pkinase=FE(11.5=100)
C2:
PF0006920=Pkinase=FE(22.7=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGCCTGCAGGATTTCCGACTA
R:
GCCTGCTGTGTACTCCATCAC
Band lengths:
178-270
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]