DreEX6036119 @ danRer10
Exon Skipping
Gene
ENSDARG00000032630 | neb
Description
nebulin [Source:ZFIN;Acc:ZDB-GENE-041111-216]
Coordinates
chr9:23070157-23080650:-
Coord C1 exon
chr9:23080588-23080650
Coord A exon
chr9:23074113-23074229
Coord C2 exon
chr9:23070157-23070264
Length
117 bp
Sequences
Splice sites
3' ss Seq
TTAATTGTTATTTTCCTAAGCCT
3' ss Score
3.66
5' ss Seq
CATGTAAGC
5' ss Score
5.61
Exon sequences
Seq C1 exon
AGTGACTGAATCTGTTTGAAGATGGCATCGGAAGAAGAGTATGATGATGATGTTGTGGAGGAG
Seq A exon
CCTGGACCTGGTGGTCTTCCTAGGTGCTCAGTCAGGAAGAAAGTGAAGGTGGACACCTCCAAGTTCATGACTCCTTACCTGGCACACAGCCAGAAGATGCTGGACCAGTACAGCCAT
Seq C2 exon
AACAAATACAGGGAGGAATATGAAAAATCAAAGGGGCAGCCCTACCTCATTTCGGCTGACACACCTGAGATGATTCGCATCAAAAAGGCCCAGGAGCAGCTAAGTGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032630-'3-5,'3-3,5-5=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.908 A=0.304 C2=0.199
Domain overlap (PFAM):
C1:
NO
A:
PF0088013=Nebulin=PU(55.2=41.0)
C2:
PF0088013=Nebulin=PD(37.9=30.6),PF0088013=Nebulin=PU(55.2=44.4)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGACTGAATCTGTTTGAAGATGGC
R:
CTCACTTAGCTGCTCCTGGGC
Band lengths:
170-287
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]