DreEX6042509 @ danRer10
Exon Skipping
Gene
ENSDARG00000077103 | sema4aa
Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4Aa [Source:ZFIN;Acc:ZDB-GENE-110411-86]
Coordinates
chr16:43148128-43149891:-
Coord C1 exon
chr16:43149750-43149891
Coord A exon
chr16:43148352-43148541
Coord C2 exon
chr16:43148128-43148243
Length
190 bp
Sequences
Splice sites
3' ss Seq
TTTCTCTCATGTTTTCACAGTGT
3' ss Score
9.74
5' ss Seq
CCGGTGAAC
5' ss Score
2.72
Exon sequences
Seq C1 exon
GTCGGTTTCATCTGGTCTGTCAGCGGTGTGTGCTTTCAGTCTGACGGATATTAAAGCTGCGTTTTCAGGGAATTACAAGACATTCGACTTGAGAGGAAATCACTGGTCACCTCAGCCAAATACAGACGGCAAACTCGGCAAG
Seq A exon
TGTGGATTATTTAATGACACTGACAGCGTGTTGAACAGAGTGAAAAAGAGCTTCCTGACCGAAAACGTTGTGCATCCAATGGATAAAACACTGCTTCTGTCCTCCGCAGAAGAGCAATATACTCGTCTAGCTGTTCACAGGGCTCAAGCTGTAGATGGACAAACTTACACCATCCTGTACCTGCTCACCG
Seq C2 exon
AGTCTGGATTTCTTCATAAAGTGGTCCTGCTTGCTGAAGGTCCTCATATCATCGAGGAGATTCAGATTTTTAAGCAGCCACAGATTGCGAAAAACATCCTCCTGTCCAAAAGCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000077103-'8-11,'8-10,9-11=AN
Average complexity
A_S
Mappability confidence:
88%=100=75%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.104 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(11.3=100)
A:
PF0140314=Sema=FE(15.2=100)
C2:
PF0140314=Sema=FE(9.2=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTCATCTGGTCTGTCAGCGGT
R:
GGACAGGAGGATGTTTTTCGCA
Band lengths:
243-433
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]