MmuEX6071974 @ mm9
Exon Skipping
Gene
ENSMUSG00000028064 | Sema4a
Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A [Source:MGI Symbol;Acc:MGI:107560]
Coordinates
chr3:88242365-88252063:-
Coord C1 exon
chr3:88251913-88252063
Coord A exon
chr3:88244620-88244800
Coord C2 exon
chr3:88242365-88242483
Length
181 bp
Sequences
Splice sites
3' ss Seq
CTGCTTCCCTCCTCCTCTAGTGC
3' ss Score
10.38
5' ss Seq
CCTGTAAGT
5' ss Score
7.52
Exon sequences
Seq C1 exon
GCAGGTTGGCGGGACCAGGAGCTCAGCAGTCTGTGCCTTCTCTCTCACGGACATTGAGCGAGTCTTTAAAGGGAAGTACAAGGAGCTGAACAAGGAGACCTCCCGCTGGACCACTTACCGGGGCTCAGAGGTCAGCCCGAGGCCAGGCAGT
Seq A exon
TGCTCCATGGGCCCCTCCTCTGACAAAGCCTTGACCTTCATGAAGGACCATTTTCTGATGGATGAGCACGTGGTAGGAACACCCCTGCTGGTGAAGTCTGGTGTGGAGTACACACGGCTTGCTGTGGAGTCAGCTCGGGGCCTTGATGGGAGCAGCCATGTGGTCATGTATCTGGGTACCT
Seq C2 exon
CCACGGGGTCCCTGCACAAGGCTGTGGTGCCTCAGGACAGCAGTGCTTATCTCGTGGAGGAGATTCAGCTGAGCCCTGACTCTGAGCCTGTTCGAAACCTGCAGCTGGCCCCCGCCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028064-'32-34,'32-33,33-34=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.216 A=0.049 C2=0.100
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(12.0=100)
A:
PF0140314=Sema=FE(14.5=100)
C2:
PF0140314=Sema=FE(12.0=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GACCAGGAGCTCAGCAGTCT
R:
CTGCAGGTTTCGAACAGGCTC
Band lengths:
243-424
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: