RnoEX6010211 @ rn6
Exon Skipping
Gene
ENSRNOG00000019737 | Sema4a
Description
semaphorin 4A [Source:RGD Symbol;Acc:1304636]
Coordinates
chr2:187801971-187810234:-
Coord C1 exon
chr2:187810084-187810234
Coord A exon
chr2:187803014-187803194
Coord C2 exon
chr2:187801971-187802089
Length
181 bp
Sequences
Splice sites
3' ss Seq
CTGCTTCCCTCCTCCGCCAGTGC
3' ss Score
8.54
5' ss Seq
CCTGTGAGT
5' ss Score
7.21
Exon sequences
Seq C1 exon
GCAGGTTGGTGGGACCAGGAGCTCAGCAGTCTGTGCCTTCTCTCTCACGGACATTGAGCGAGTCTTTAAAGGAAAGTACAAGGAGCTGAACAAAGAAACCTCCCGCTGGACCACTTACAGGGGCTCAGAGGTCAACCCGAGGCCAGGCAGC
Seq A exon
TGCTCCATGGGCCCCTCCTCTGACAAAGCCTTGACCTTCATGAAGGACCATTTTCTGATGGATGAGCATGTGGTAGGAGCTCCCCTGCTGGTGAAGTCTGGCGTGGAGTACACACAGCTTGCCGTGGAAACAGCTCAGGGCCTTGACGGGAGCAGCCATGTGGTCCTTTACCTGGGTACCT
Seq C2 exon
CTACGGGGTCCCTGCACAAAGCTGTGGTGCCTCAGAATAGCAGTGCTTATCTTGTGGAGGAGATTCAGCTGAGCCCTAACTCTGAGCCTGTTCAGAACCTGCAGCTGGCCCCCACCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000019737-'22-29,'22-28,24-29=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.235 A=0.066 C2=0.138
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(12.0=100)
A:
PF0140314=Sema=FE(14.5=100)
C2:
PF0140314=Sema=FE(9.4=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GACCAGGAGCTCAGCAGTCT
R:
CTGCAGGTTCTGAACAGGCTC
Band lengths:
243-424
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]