DreEX6044248 @ danRer10
Exon Skipping
Gene
ENSDARG00000032238 | dnm3a
Description
dynamin 3a [Source:ZFIN;Acc:ZDB-GENE-040724-76]
Coordinates
chr20:14939441-14947698:-
Coord C1 exon
chr20:14947534-14947698
Coord A exon
chr20:14944422-14944657
Coord C2 exon
chr20:14939441-14939596
Length
236 bp
Sequences
Splice sites
3' ss Seq
TTTCTGTTCTTTACCAATAGGTA
3' ss Score
11.36
5' ss Seq
CAGGTACCT
5' ss Score
8.16
Exon sequences
Seq C1 exon
GCAGATGGAGATGGTTCAAGCCAGACGGAGAGCTTCTCTATGGACCCTCAGTTGGAGCGTCAGGTCGAGACCATCCGCAACCTGGTGGACTCCTACATGAGCATCGTCTACAAAAGCATCCGAGACCTGATGCCCAAGACCATCATGCATCTTGTCATCAACAAT
Seq A exon
GTAGAAGAGTTCATCCACTCTGAGTTGCTGGCTCAGTTGTATTCTTCAGGAGATCAGTACTCTCTGATGGACGAGTCTCCTGAGCAAGCCTTGCGCAGAGAGGAGGTTCTGCGTACCCATGCTGCCCTGAAAGAGGCTCTGAACATCATCACTGACATCTCCACCACCACCATCTCCACCCCGCTGCCACCTCCCGTAGATAACTCCTGGCTATCCAGTGGCTCGCTTCACCGCAG
Seq C2 exon
GTCTCCTAAAGGTTATAGTGTCCCTAAGAAGCATCCAGCTCCAGCTGCTCCATCTCAACACGTTCGCTTGGAAGCTCAGCCTCTACCTGTCTACAACAACAAAGATAACACCAACACCACCAGCCATCCCAAACGAGTCCCTCCTAGTGTTCCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032238-'26-29,'26-28,27-29=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.309 A=0.407 C2=1.000
Domain overlap (PFAM):
C1:
PF0061614=RasGAP=FE(38.0=100),PF0221213=GED=PU(41.3=69.1)
A:
PF0061614=RasGAP=PD(4.2=7.6),PF0221213=GED=PD(56.5=65.8)
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Cow
(bosTau6)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCCAGACGGAGAGCTTCTCTA
R:
CTGGGAACACTAGGAGGGACT
Band lengths:
302-538
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]