DreEX6050299 @ danRer10
Exon Skipping
Gene
ENSDARG00000011672 | sema3b
Description
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B [Source:ZFIN;Acc:ZDB-GENE-081105-78]
Coordinates
chr8:26574723-26575250:-
Coord C1 exon
chr8:26575108-26575250
Coord A exon
chr8:26574897-26575011
Coord C2 exon
chr8:26574723-26574792
Length
115 bp
Sequences
Splice sites
3' ss Seq
TTGTTTTTTTTTTGTACCAGAAT
3' ss Score
8.59
5' ss Seq
TCCGTAAGA
5' ss Score
6.41
Exon sequences
Seq C1 exon
AGCCCAAGTTTATTGCAGCATTTGGTGTTCCTGAGAGTGATAACCCTGACGACGATAAAGTCTTCTTCTTCTTCAGGGAAACTGCTGTAGAAGCTCAGGGATTTGGGAAGGTTACTTATTCCCGGATTGGTCAGCTCTGCAGG
Seq A exon
AATGATATGGGAGGACAGCGCAGTCTAGTAAACAAGTGGACAACATTTCTAAAGGCTCGTCTTGTGTGTTCTGTACCAGGCAATGATGGCACTGAAACGCATTTTGATGAACTCC
Seq C2 exon
GGGACGTTTTCTTACTGCAGACCCGTGACAAAAAGAACCCGCTTATCTACACCGTCTTTACCACCTCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000011672-'8-12,'8-11,9-12=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.021 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(10.6=100)
A:
PF0140314=Sema=FE(8.6=100)
C2:
PF0140314=Sema=FE(5.2=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGCCCAAGTTTATTGCAGCAT
R:
TGGAGGTGGTAAAGACGGTGT
Band lengths:
212-327
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]