DreEX6058102 @ danRer10
Exon Skipping
Gene
ENSDARG00000004078 | acsl4a
Description
acyl-CoA synthetase long-chain family member 4a [Source:ZFIN;Acc:ZDB-GENE-040426-1565]
Coordinates
chr14:28147145-28148761:+
Coord C1 exon
chr14:28147145-28147336
Coord A exon
chr14:28148357-28148471
Coord C2 exon
chr14:28148604-28148761
Length
115 bp
Sequences
Splice sites
3' ss Seq
ATTTGATCTGTAACCTCTAGACC
3' ss Score
6.51
5' ss Seq
CAGGTTTGC
5' ss Score
4.86
Exon sequences
Seq C1 exon
GTGGTTATACAAAGCGTGACAAGCCTCATCCGCGTGGCGAGATCCTCATCGGTGGTCCTAATGTGACTATGGGCTACTACAGGACTGAAGGCTGTAATAATGACGACTTCTTTGTCGATCAGAATGGCCAGCGGTGGTTCTGCACTGGCGATATAGGAGAGATGCACGCTGACGGCTGTCTACAGATTGTTG
Seq A exon
ACCGCAAGAAGGATCTTGTAAAACTTCAAGCTGGAGAGTACGTCTCTTTGGGCAAAGTTGAGTCAGCGCTGAAGAACTGCTCTTTCGTTGACAACATCTGCGCTTACGCAAACAG
Seq C2 exon
TGACCAGAACTACGTTATCAGCTTCATTGTTCCTAATCAGAAGAAACTGACAGCTCTTGCCAATCAGAAAGGCATTGAAGGCACGTGGGAAGAAATCTGTAACCACCCAGTTATGGAAAGGGAAGTTCTGAGAGAAATTAAAGAAGTCGCAAATTCAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000004078-'19-19,'19-18,20-19=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(13.4=100)
A:
PF0050123=AMP-binding=PD(1.9=23.1),PF131931=AMP-binding_C=PU(20.6=51.3)
C2:
PF131931=AMP-binding_C=FE(54.6=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGATCCTCATCGGTGGTCCT
R:
ACAGATTTCTTCCCACGTGCC
Band lengths:
254-369
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]