Special

MmuEX6046228 @ mm10

Exon Skipping

Gene
ENSMUSG00000031278 | Acsl4
Description
acyl-CoA synthetase long-chain family member 4 [Source:MGI Symbol;Acc:MGI:1354713]
Coordinates
chrX:142327976-142332383:-
Coord C1 exon
chrX:142332192-142332383
Coord A exon
chrX:142328848-142328962
Coord C2 exon
chrX:142327976-142328133
Length
115 bp
Sequences
Splice sites
3' ss Seq
CCATATTTTTATGATTCTAGATC
3' ss Score
6.63
5' ss Seq
AAGGTAACT
5' ss Score
9.01
Exon sequences
Seq C1 exon
GTGGTTATACAGTTCATGATAAGCCGAACCCCAGAGGTGAGATTGTGATCGGTGGCCAGAATATCTCCATGGGATATTTTAAAAACGAAGAGAAAACAGCAGAAGATTATTGTGTTGATGAAAATGGACAAAGGTGGTTTTGCACTGGCGATATTGGAGAATTCCATCCTGATGGATGCTTACAGATTATAG
Seq A exon
ATCGTAAGAAAGATCTGGTAAAGTTACAAGCAGGAGAATATGTATCTCTTGGGAAAGTAGAAGCTGCACTGAAGAATTGTCCACTGATCGACAACATCTGTGCTTTTGCCAAAAG
Seq C2 exon
TGACCAGTCCTATGTGATCAGTTTTGTGGTTCCTAACCAGAAAAAGTTGACTCTTTTGGCACAACAGAAGGGGGTAGAAGGATCTTGGGTTGATATTTGCAATAATCCCGCCATGGAAGCTGAAATACTGAAAGAAATTCGAGAAGCTGCAAATGCCA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000031278-'23-26,'23-25,24-26=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.015 A=0.000 C2=0.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0050123=AMP-binding=FE(13.4=100)

							
A:
PF0050123=AMP-binding=PD(1.9=23.1),PF131931=AMP-binding_C=PU(20.6=51.3)

							
C2:
PF131931=AMP-binding_C=FE(54.6=100)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000033634





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000108524, ENSMUSP00000108525, ENSMUSP00000108528
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm9




Conservation


Human
(hg38)
chrX:109661531-109661645 
HIGH PSI
HsaEX6052914
(ACSL4)
Human
(hg19)
chrX:108904760-108904874 
HIGH PSI
HsaEX6052914
(ACSL4)
Rat
(rn6)
chrX:113605214-113605328 
HIGH PSI
RnoEX6026662
(Acsl4)
Cow
(bosTau6)
chrX:62486168-62486282 
HIGH PSI
BtaEX6006050
(ACSL4)
Chicken
(galGal4)
chr4:13753013-13753127 
HIGH PSI
GgaEX6033623
(ACSL4)
Chicken
(galGal3)
chr4:13895793-13895907 
HIGH PSI
GgaEX6033623
(ACSL4)
Zebrafish
(danRer10)
chr14:28148357-28148471 
HIGH PSI
DreEX6058102
(acsl4a)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GAGATTGTGATCGGTGGCCAG

				
R: 
ACCCAAGATCCTTCTACCCCC

				
Band lengths: 
244-359

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types





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