DreEX6063029 @ danRer10
Exon Skipping
Gene
ENSDARG00000052702 | capn1a
Description
calpain 1, (mu/I) large subunit a [Source:ZFIN;Acc:ZDB-GENE-040426-1263]
Coordinates
chr13:42566857-42572756:-
Coord C1 exon
chr13:42572682-42572756
Coord A exon
chr13:42569901-42570061
Coord C2 exon
chr13:42566857-42567026
Length
161 bp
Sequences
Splice sites
3' ss Seq
ATGTGTGGTTTTGTCCACAGGAT
3' ss Score
11.53
5' ss Seq
CCAGTGAGT
5' ss Score
8.28
Exon sequences
Seq C1 exon
CTCTCCTGAATGGGATGAGATTGATCCATCAGAGAAGGACGACTTGCACTTACAAATGGAGGATGGAGAGTTTTG
Seq A exon
GATGTCATTTGGTGAGTTTTTACGTCAGTTCTCCCGGCTGGAGATTTGCAATCTGACCCCTGATGCTCTGAGTGACGATGATATGAGCCACTGGAACACCATCAAGTTTCATGGAGCCTGGAGGAGAGGAAGCACTGCAGGAGGATGCCGAAATCATCCCA
Seq C2 exon
ACACGTTCTGGATCAATCCACAGTATAAGATCACACTGCTGGAGGAAGATGATGATCCGGAGGATGAAGAAGTGGCCTGCAGCTTCCTGGTGGCTTTAATGCAGAAAGACCGCAGACGGTACCGCCGCCACGGACAAGATATGCACACTATCGGTTTTGCAATTTATGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000052702-'10-15,'10-13,11-15=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.365 A=0.000 C2=0.035
Domain overlap (PFAM):
C1:
PF0064816=Peptidase_C2=FE(8.3=100)
A:
PF0064816=Peptidase_C2=PD(6.3=34.5),PF0106717=Calpain_III=PU(15.4=43.6)
C2:
PF0106717=Calpain_III=FE(35.9=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTCTCCTGAATGGGATGAGATTGA
R:
ATAAATTGCAAAACCGATAGTGTGCA
Band lengths:
242-403
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]