DreEX6066459 @ danRer10
Exon Skipping
Gene
ENSDARG00000003420 | chrna5
Description
cholinergic receptor, nicotinic, alpha 5 [Source:ZFIN;Acc:ZDB-GENE-050417-440]
Coordinates
chr18:40502731-40503874:-
Coord C1 exon
chr18:40503830-40503874
Coord A exon
chr18:40503604-40503713
Coord C2 exon
chr18:40502731-40502908
Length
110 bp
Sequences
Splice sites
3' ss Seq
TCAATCTCTTTCACATTCAGGAA
3' ss Score
10.2
5' ss Seq
CAAGTATGT
5' ss Score
7.44
Exon sequences
Seq C1 exon
GATGAGAAGAACCAGCTGATGACGACCAATGTGTGGATGAAGCAG
Seq A exon
GAATGGACAGACTTGAAGCTCAGATGGGATCCGGATCATTACCTGGGCATCACCTCTATCAGGGTGCCTTCAGACTCCATCTGGATTCCTGACATTGTGCTCTATGACAA
Seq C2 exon
TGCAGATGGTAATTTCGAGGCCACAGTGACAAAAGCAGTGGTGAGATTTGATGGCACAATCTCCTGGACTCCTCCTGCCAACTATAAATCTGCCTGCACCATTGATGTGACCTTCTTCCCCTTTGACCTTCAAAACTGCTCCATGAAGTTCGGTTCTTGGACCTATGATGGCTCCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000003420-'6-8,'6-7,7-8=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0293118=Neur_chan_LBD=FE(6.9=100)
A:
PF0293118=Neur_chan_LBD=FE(17.6=100)
C2:
PF0293118=Neur_chan_LBD=FE(28.9=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGAGAAGAACCAGCTGATGACG
R:
GAGCCATCATAGGTCCAAGAACC
Band lengths:
218-328
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]