DreEX6067631 @ danRer10
Exon Skipping
Gene
ENSDARG00000099954 | plekhm1
Description
pleckstrin homology domain containing, family M (with RUN domain) member 1 [Source:ZFIN;Acc:ZDB-GENE-030131-2752]
Coordinates
chr12:4893139-4910365:+
Coord C1 exon
chr12:4893139-4893227
Coord A exon
chr12:4900700-4900944
Coord C2 exon
chr12:4909891-4910365
Length
245 bp
Sequences
Splice sites
3' ss Seq
TCTCTCTCTGTACCCTGCAGTGG
3' ss Score
8.39
5' ss Seq
CAGGTGGGC
5' ss Score
8.07
Exon sequences
Seq C1 exon
TGTGTGAAGTGGAAATAATCACAGCTAGAGGAGCCATGCTGGCCACCCAAACACCTGAGAGCGGCCCAGACGCTAAAGATGTTAAACAG
Seq A exon
TGGATCAAGGGCAAGATCGCACATACATTAAAAGCCCTGCAGAAGCGCTACATCACCACAGACACGGCGGTCACCAGTGAGGATCTAGAAGCAAACCTGCTGTGCTGTGCTTTGGAGGCGGTTTTCATCCATGGAATCAAGAGTAAGTTCATCCGATCTGATGGAGGTCAAGTGCGCAAAGGAGGATCTCGAGGAGCGCTGCCTCAGCCAGTCTTCTGGGCTCTGTTGAAGACTGTCACCCACAG
Seq C2 exon
AGAGAGCCTGATGAAGGAGGACATTGATGTGTTCATACCAGCTCCAGACAATCTGACTGACACGCATTCTGTCAACGAGCACACGCACATACCTGACGCCTCACTGACATCAGCACAGGACACGCATCACTCGCACATCACAGAGCAATCTGTAGAGAACATATCAGTGGAGACGAAAGAAGAAACAATAGAAGAATCTGAGATTATAGTTAAAAGCACAAGACTTAAAGCTAAAAATACTGTGAGTGAGACTGCAGAGAAGGAGAAGAAGAGGGAGAGCAGAGACTCATCTGTTGATCCTCCTTCAGTTTCTGCTTTAACAGAGACTGAGGAGACGCCTGACCCTAAAGACACTGTTCTTTCAGAAATTTCTGCTGCTCCTAGTCCAGTGGAAACACCTCAACATGCTGCATGCAGACGCACCACAATGAGCGTCAGCAGGAAAATATCCACAGACTCGCTCTATTCCTCCTGT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000099954-'2-5,'2-3,4-5=AN
Average complexity
A_C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.611 A=0.037 C2=0.862
Domain overlap (PFAM):
C1:
NO
A:
PF0275914=RUN=PU(36.8=59.8)
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGCCCAGACGCTAAAGATGTT
R:
TGCTCTCCCTCTTCTTCTCCT
Band lengths:
308-553
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]