DreEX6073710 @ danRer10
Exon Skipping
Gene
ENSDARG00000028533 | macf1a
Description
microtubule-actin crosslinking factor 1a [Source:ZFIN;Acc:ZDB-GENE-030131-3606]
Coordinates
chr19:35547935-35548638:-
Coord C1 exon
chr19:35548447-35548638
Coord A exon
chr19:35548190-35548366
Coord C2 exon
chr19:35547935-35548058
Length
177 bp
Sequences
Splice sites
3' ss Seq
TCCTGTGTTTCTACTTTCAGTCT
3' ss Score
9.69
5' ss Seq
CAGGTAGGA
5' ss Score
9.79
Exon sequences
Seq C1 exon
CTTGCAGAAGATGTCATCTGTCACAAAGCAGATCTGCGCTTTGTCACTATCTCTGGGCAGAAGGTTCTGGATTCTGTCCAGTGTGCCCTGCAGAGGATGGGCAGTGCAGATCCGGCTCTGGAGGAAACCAAAAATCTAGTCAGTGACAAGCTCCAAGATGCCACACAGCGCTACGGTTCATTACATAGCAAG
Seq A exon
TCTTCAGAACTTGGCACCCGCCTCTCAAGTCTCCAGGAGCGATACCAGCACTACCAAGATGAGGCTGGTTCTTTAGACTCGTGGCTGAACTCTCAGGAGCAGAACCAGAGTATATTTAAGCCCAGCGGAGAACAAACTGACCCTCAGGCCTTGCAGCGCACTCTCAGTACTGTACAG
Seq C2 exon
CTGCTGCAGGATGATTTGGCAGAGCGTTCAGTACAGCTTGAGAAGGTCAAGAGGGCAGGGAAAGAGCTGGTCAGCATTAAGGAGTCCCCAACTCTCAAGAATGCAGATATTAAGAATGTGACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000028533-'54-59,'54-58,55-59=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.312 A=0.390 C2=0.024
Domain overlap (PFAM):
C1:
NO
A:
PF0043516=Spectrin=PU(41.4=78.0)
C2:
PF0043516=Spectrin=FE(36.9=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGCTTTGTCACTATCTCTGGGC
R:
GGGGACTCCTTAATGCTGACC
Band lengths:
245-422
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]